E-ISSN 2229-7723 | ISSN 0976-9234
 

Original Article 


Antimicrobial effect of chloroform Hibiscus sabdariffa extract on pathogenic bacteria

Elaheh Aleebrahim-Dehkordy, Mahmoud Rafieian-Kopaei, Behnam Zamanzad, Fatemeh Deris, Abolghasem Sharifih, Ali Reyhanian.

Abstract
Objective: The aim of this study was to determine the antimicrobial effect of Hibiscus sabdariffa extract on oral and dental bacterial pathogens. Materials and Methods: In this study, the extract of H. sabdariffa was isolated by maceration with chloroform and concentrated by a rotary evaporator. The antimicrobial effects were examined using broth microdilution, and minimum inhibitory concentration and minimum bactericidal concentration values were determined. Next, the effect of different concentrations of extract on the growth of Streptococcus pyogenes, Streptococcus mutans, and Streptococcus salivarius was investigated by disc diffusion method. Finally, the data were analyzed using SPSS 16 software (SPSS Inc., 233 South Wacker Drive, 11th Floor, Chicago, IL 60606-6412), and the results were presented as mean (± standard deviation). Results: The results of this study showed that the chloroform extract of H. sabdariffa had inhibitory and bactericidal effects on three bacteria in both broth microdilution and disc diffusion methods. In broth microdilution, S. mutans and S. salivarius were more resistant to H. sabdariffa extract than S. pyogenes. In disc diffusion method, the highest inhibitory effect of the extract was observed on S. pyogenes at 25 μg/ml with a growth inhibition zone diameter of 13.03 ± 0.15 mm. Conclusion: According to the results of this study, it can be argued that the chloroform extract of H. sabdariffa is effective in inhibiting S. pyogenes, S. mutans, and S. salivarius in both broth microdilution and disc diffusion methods.

Key words: Antibiotic, antimicrobial effect, chloroform extract, Hibiscus sabdariffa, oral cavity and teeth


 
ARTICLE TOOLS
Abstract
PDF Fulltext
How to cite this articleHow to cite this article
Citation Tools
Related Records
 Articles by Elaheh Aleebrahim-Dehkordy
Articles by Mahmoud Rafieian-Kopaei
Articles by Behnam Zamanzad
Articles by Fatemeh Deris
Articles by Abolghasem Sharifih
Articles by Ali Reyhanian
on Google
on Google Scholar


How to Cite this Article
Pubmed Style

Elaheh Aleebrahim-Dehkordy, Mahmoud Rafieian-Kopaei, Behnam Zamanzad, Fatemeh Deris, Abolghasem Sharifih, Ali Reyhanian. Antimicrobial effect of chloroform Hibiscus sabdariffa extract on pathogenic bacteria. PNR. 2020; 11(1): 15-18. doi:10.4103/jpnr.JPNR_19_18


Web Style

Elaheh Aleebrahim-Dehkordy, Mahmoud Rafieian-Kopaei, Behnam Zamanzad, Fatemeh Deris, Abolghasem Sharifih, Ali Reyhanian. Antimicrobial effect of chloroform Hibiscus sabdariffa extract on pathogenic bacteria. http://www.pnrjournal.com/?mno=133356 [Access: January 26, 2022]. doi:10.4103/jpnr.JPNR_19_18


AMA (American Medical Association) Style

Elaheh Aleebrahim-Dehkordy, Mahmoud Rafieian-Kopaei, Behnam Zamanzad, Fatemeh Deris, Abolghasem Sharifih, Ali Reyhanian. Antimicrobial effect of chloroform Hibiscus sabdariffa extract on pathogenic bacteria. PNR. 2020; 11(1): 15-18. doi:10.4103/jpnr.JPNR_19_18



Vancouver/ICMJE Style

Elaheh Aleebrahim-Dehkordy, Mahmoud Rafieian-Kopaei, Behnam Zamanzad, Fatemeh Deris, Abolghasem Sharifih, Ali Reyhanian. Antimicrobial effect of chloroform Hibiscus sabdariffa extract on pathogenic bacteria. PNR. (2020), [cited January 26, 2022]; 11(1): 15-18. doi:10.4103/jpnr.JPNR_19_18



Harvard Style

Elaheh Aleebrahim-Dehkordy, Mahmoud Rafieian-Kopaei, Behnam Zamanzad, Fatemeh Deris, Abolghasem Sharifih, Ali Reyhanian (2020) Antimicrobial effect of chloroform Hibiscus sabdariffa extract on pathogenic bacteria. PNR, 11 (1), 15-18. doi:10.4103/jpnr.JPNR_19_18



Turabian Style

Elaheh Aleebrahim-Dehkordy, Mahmoud Rafieian-Kopaei, Behnam Zamanzad, Fatemeh Deris, Abolghasem Sharifih, Ali Reyhanian. 2020. Antimicrobial effect of chloroform Hibiscus sabdariffa extract on pathogenic bacteria. Journal of Pharmaceutical Negative Results, 11 (1), 15-18. doi:10.4103/jpnr.JPNR_19_18



Chicago Style

Elaheh Aleebrahim-Dehkordy, Mahmoud Rafieian-Kopaei, Behnam Zamanzad, Fatemeh Deris, Abolghasem Sharifih, Ali Reyhanian. "Antimicrobial effect of chloroform Hibiscus sabdariffa extract on pathogenic bacteria." Journal of Pharmaceutical Negative Results 11 (2020), 15-18. doi:10.4103/jpnr.JPNR_19_18



MLA (The Modern Language Association) Style

Elaheh Aleebrahim-Dehkordy, Mahmoud Rafieian-Kopaei, Behnam Zamanzad, Fatemeh Deris, Abolghasem Sharifih, Ali Reyhanian. "Antimicrobial effect of chloroform Hibiscus sabdariffa extract on pathogenic bacteria." Journal of Pharmaceutical Negative Results 11.1 (2020), 15-18. Print. doi:10.4103/jpnr.JPNR_19_18



APA (American Psychological Association) Style

Elaheh Aleebrahim-Dehkordy, Mahmoud Rafieian-Kopaei, Behnam Zamanzad, Fatemeh Deris, Abolghasem Sharifih, Ali Reyhanian (2020) Antimicrobial effect of chloroform Hibiscus sabdariffa extract on pathogenic bacteria. Journal of Pharmaceutical Negative Results, 11 (1), 15-18. doi:10.4103/jpnr.JPNR_19_18





Most Viewed Articles
  • Pharmacoepidemiology of Antiepileptic Drugs in Children
    Darya Knyazeva, Yulia Gushchina, Oksana Zakharova
    PNR. 2021; 12(1): 1-6
    » Abstract » doi: 10.47750/pnr.2021.12.01.001

  • Comparative Benefits of Statins in Coronary Heart Disease
    Viktoriya Ermakova, Hizir Bataev, Natalya Semenenko
    PNR. 2021; 12(1): 7-13
    » Abstract » doi: 10.47750/pnr.2021.12.01.002

  • Influence of polylactic acid and polycaprolactone on dissolution characteristics of ansamycin-loaded polymeric nanoparticles: An unsatisfied attempt for drug release profile
    Keerthi GS Nair, Ramaiyan Velmurugan, Sathesh Kumar Sukumaran
    PNR. 2020; 11(1): 23-29
    » Abstract » doi: 10.4103/jpnr.JPNR_26_19

  • Oxidative stress biomarkers in bipolar disorder with suicidal behavior: A systematic review
    Nurul Ain Mohamad Kamal, Jiann Lin Loo, Jo Aan Goon, Hanafi Ahmad Damanhuri, Shalisah Sharip, Suriati Mohamed Saini, Jemaima Che Hamzah, Lai Fong Chan
    PNR. 2019; 10(1): 6-15
    » Abstract » doi: 10.4103/jpnr.JPNR_3_19

  • Ethical Issues of Decision Making and Communication in Health Care: An Intercultural and Interfaith Perspective
    Elena Grunt, Alexander Ivanov, Elena Zubko, Vladimir Starostin
    PNR. 2021; 12(1): 27-32
    » Abstract » doi: 10.47750/pnr.2021.12.01.005

  • Most Downloaded
  • Lack of activity of rutin isolated from Tontelea micrantha leaves against Vero and BHK, fungi, bacteria and Mayaro virus and its in silico activity
    Waleska Stephanie Da Cruz Nizer, Ariane Coelho Ferraz, Thais De Fatima Silva Moraes, Fernanda Lopes Ferreira, Cintia Lopes De Brito Magalhães, Sidney Augusto Vieira-Filho, Lucenir Pains Duarte, José Carlos De Magalhães
    PNR. 2020; 11(1): 9-14
    » Abstract » doi: 10.4103/jpnr.JPNR_12_19

  • No association between isocitrate dehydrogenase 1 mutation and increased survival of glioblastoma: A meta-analysis
    Thara Tunthanathip, Kantapong Mamueang, Nichapat Nilbupha, Chattarin Maliwan, Tanan Bejrananda
    PNR. 2020; 11(1): 1-8
    » Abstract » doi: 10.4103/jpnr.JPNR_22_19

  • Neurobehavioral activity study of methanolic whole plants extract of Cyperus rotundus Linn.
    Imonul Kabir, Subir Biswas, Md Asaduzzaman, Md Islam Molla, Md Rajdoula Rafe
    PNR. 2019; 10(1): 36-40
    » Abstract » doi: 10.4103/jpnr.JPNR_11_19

  • Antimicrobial effect of chloroform Hibiscus sabdariffa extract on pathogenic bacteria
    Elaheh Aleebrahim-Dehkordy, Mahmoud Rafieian-Kopaei, Behnam Zamanzad, Fatemeh Deris, Abolghasem Sharifih, Ali Reyhanian
    PNR. 2020; 11(1): 15-18
    » Abstract » doi: 10.4103/jpnr.JPNR_19_18

  • Antimicrobial resistance and presence of Class 1 integrons in Pseudomonas aeruginosa isolates from burn and wound infections
    Ali Hassoun Hammadi, Qutaiba Ahmed Al Khames Aga, Nabil Awni Nimer, Pottathil Shinu, Anroop B Nair
    PNR. 2020; 11(1): 19-22
    » Abstract » doi: 10.4103/jpnr.JPNR_9_20

  • Most Cited Articles