E-ISSN 2229-7723 | ISSN 0976-9234
 

Original Article 


Evaluation of anticancer potential of Eleusine indica methanolic leaf extract through Ras- and Wnt-related pathways using transgenic Caenorhabditis elegans strains

John Sylvester B Nas, Sheryl E Dangeros, Princess Dianne R Chen, Rosemarie C Dimapilis, Daniel Joshua G Gonzales, Fatima Jeda A Hamja, Cathdrin Joyce Ramos, Ashera D Villanueva.

Abstract
Background: In the Philippines, many accounts have resurfaced claiming different herbal and therapeutic advantages of Eleusine indica. One of these advantages is its anticancer potential. Despite some studies showing that the crude extract has cytotoxic and radical scavenging activity, it is still insufficient and further scientific evidence is needed to support this claim. Aim: Hence, we evaluate the anticancer potential of E. indic a methanolic leaf extract (EMLE) by focusing on two cancer-related pathways, Ras and Wnt pathways. Subjects and Methods: We used wild-type and transgenic Caenorhabditis elegans strains which have an irregular Ras or Wnt signaling. We determined the average number of eggs laid of each strain and the multivulva development of the Ras-mutant strain. Results: Our experiments show that EMLE does not affect the number of eggs laid of the wild-type, Ras-mutant-and Wnt-mutant worms. Furthermore, EMLE was not able to reduce Ras-mutant population demonstrating multi-vulva. Conclusion: Taken together, our data suggest that the anticancer potential of EMLE may be independent of Ras and Wnt signaling pathways.

Key words: Anticancer, Eleusine indica, ras pathway, wnt pathway


 
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How to Cite this Article
Pubmed Style

John Sylvester B Nas, Sheryl E Dangeros, Princess Dianne R Chen, Rosemarie C Dimapilis, Daniel Joshua G Gonzales, Fatima Jeda A Hamja, Cathdrin Joyce Ramos, Ashera D Villanueva. Evaluation of anticancer potential of Eleusine indica methanolic leaf extract through Ras- and Wnt-related pathways using transgenic Caenorhabditis elegans strains. pnr. 2020; 11(1): 42-46. doi:10.4103/jpnr.JPNR_7_20


Web Style

John Sylvester B Nas, Sheryl E Dangeros, Princess Dianne R Chen, Rosemarie C Dimapilis, Daniel Joshua G Gonzales, Fatima Jeda A Hamja, Cathdrin Joyce Ramos, Ashera D Villanueva. Evaluation of anticancer potential of Eleusine indica methanolic leaf extract through Ras- and Wnt-related pathways using transgenic Caenorhabditis elegans strains. http://www.pnrjournal.com/?mno=133385 [Access: September 22, 2020]. doi:10.4103/jpnr.JPNR_7_20


AMA (American Medical Association) Style

John Sylvester B Nas, Sheryl E Dangeros, Princess Dianne R Chen, Rosemarie C Dimapilis, Daniel Joshua G Gonzales, Fatima Jeda A Hamja, Cathdrin Joyce Ramos, Ashera D Villanueva. Evaluation of anticancer potential of Eleusine indica methanolic leaf extract through Ras- and Wnt-related pathways using transgenic Caenorhabditis elegans strains. pnr. 2020; 11(1): 42-46. doi:10.4103/jpnr.JPNR_7_20



Vancouver/ICMJE Style

John Sylvester B Nas, Sheryl E Dangeros, Princess Dianne R Chen, Rosemarie C Dimapilis, Daniel Joshua G Gonzales, Fatima Jeda A Hamja, Cathdrin Joyce Ramos, Ashera D Villanueva. Evaluation of anticancer potential of Eleusine indica methanolic leaf extract through Ras- and Wnt-related pathways using transgenic Caenorhabditis elegans strains. pnr. (2020), [cited September 22, 2020]; 11(1): 42-46. doi:10.4103/jpnr.JPNR_7_20



Harvard Style

John Sylvester B Nas, Sheryl E Dangeros, Princess Dianne R Chen, Rosemarie C Dimapilis, Daniel Joshua G Gonzales, Fatima Jeda A Hamja, Cathdrin Joyce Ramos, Ashera D Villanueva (2020) Evaluation of anticancer potential of Eleusine indica methanolic leaf extract through Ras- and Wnt-related pathways using transgenic Caenorhabditis elegans strains. pnr, 11 (1), 42-46. doi:10.4103/jpnr.JPNR_7_20



Turabian Style

John Sylvester B Nas, Sheryl E Dangeros, Princess Dianne R Chen, Rosemarie C Dimapilis, Daniel Joshua G Gonzales, Fatima Jeda A Hamja, Cathdrin Joyce Ramos, Ashera D Villanueva. 2020. Evaluation of anticancer potential of Eleusine indica methanolic leaf extract through Ras- and Wnt-related pathways using transgenic Caenorhabditis elegans strains. Journal of Pharmaceutical Negative Results, 11 (1), 42-46. doi:10.4103/jpnr.JPNR_7_20



Chicago Style

John Sylvester B Nas, Sheryl E Dangeros, Princess Dianne R Chen, Rosemarie C Dimapilis, Daniel Joshua G Gonzales, Fatima Jeda A Hamja, Cathdrin Joyce Ramos, Ashera D Villanueva. "Evaluation of anticancer potential of Eleusine indica methanolic leaf extract through Ras- and Wnt-related pathways using transgenic Caenorhabditis elegans strains." Journal of Pharmaceutical Negative Results 11 (2020), 42-46. doi:10.4103/jpnr.JPNR_7_20



MLA (The Modern Language Association) Style

John Sylvester B Nas, Sheryl E Dangeros, Princess Dianne R Chen, Rosemarie C Dimapilis, Daniel Joshua G Gonzales, Fatima Jeda A Hamja, Cathdrin Joyce Ramos, Ashera D Villanueva. "Evaluation of anticancer potential of Eleusine indica methanolic leaf extract through Ras- and Wnt-related pathways using transgenic Caenorhabditis elegans strains." Journal of Pharmaceutical Negative Results 11.1 (2020), 42-46. Print. doi:10.4103/jpnr.JPNR_7_20



APA (American Psychological Association) Style

John Sylvester B Nas, Sheryl E Dangeros, Princess Dianne R Chen, Rosemarie C Dimapilis, Daniel Joshua G Gonzales, Fatima Jeda A Hamja, Cathdrin Joyce Ramos, Ashera D Villanueva (2020) Evaluation of anticancer potential of Eleusine indica methanolic leaf extract through Ras- and Wnt-related pathways using transgenic Caenorhabditis elegans strains. Journal of Pharmaceutical Negative Results, 11 (1), 42-46. doi:10.4103/jpnr.JPNR_7_20