E-ISSN 2229-7723 | ISSN 0976-9234
 

Original Article 


Absence of the antibacterial activity of the crude extracts and compounds isolated from M. rubiginosa against extended-spectrum β-lactamase producing enterobacteria

Geisiany M de Queiroz, Maria Gorete M de Souza, Tatiane C de Carvalho, Luciana A Casemiro, Wilson R Cunha, Carlos H. G. Martins.

Abstract
Objective: The present study evaluates the antibacterial activity (Minimum Inhibitory Concentration- MIC, Minimum Bactericidal Concentration- MBC) and the Fractional Inhibitory Concentration (FIC) of crude extracts and compounds isolated from Miconia rubiginosa against ten clinical bacterial isolates and one standard bacterial strain Expanded-Spectrum β-lactamase (ESBL)-producing enterobacteria. Materials and Methods: The crude extracts were obtained by maceration and the compounds isolated were purified through different chromatography techniques, and then structures were identified for physical methods of organic analysis. The evaluation of the antibacterial activity for the microdiluition in broth technique. Results: The MIC and MBC result for the ethanolic extract was 2.0 mg/mL. For the dichloromethane extract and ursolic acid and oleanolic acid, MIC was >2.0 mg/mL, but no bactericidal activity (MBC) was observed. The FIC values achieved with the combinatioin of the crude extracts with clavulanic acid were not significantly different. Conclusion: The ethanolic extract from Miconia rubiginosa exhibits better antibacterial activity, but the two isolated compounds are not active against the tested bacterial isolates. The combination of the crude extracts with clavulanic acid does not lead to synergism, and there are no statistical differences between the two crude extracts in this sense.

Key words: Antibacterial activity, Beta-lactamase, Miconia rubiginosa, oleanolic acid, ursolic acid


 
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How to Cite this Article
Pubmed Style

Geisiany M de Queiroz, Maria Gorete M de Souza, Tatiane C de Carvalho, Luciana A Casemiro, Wilson R Cunha, Carlos H. G. Martins. Absence of the antibacterial activity of the crude extracts and compounds isolated from M. rubiginosa against extended-spectrum β-lactamase producing enterobacteria. pnr. 2011; 2(1): 1-7. doi: 10.4103/0976-9234.82982


Web Style

Geisiany M de Queiroz, Maria Gorete M de Souza, Tatiane C de Carvalho, Luciana A Casemiro, Wilson R Cunha, Carlos H. G. Martins. Absence of the antibacterial activity of the crude extracts and compounds isolated from M. rubiginosa against extended-spectrum β-lactamase producing enterobacteria. http://www.pnrjournal.com/?mno=133439 [Access: September 22, 2020]. doi: 10.4103/0976-9234.82982


AMA (American Medical Association) Style

Geisiany M de Queiroz, Maria Gorete M de Souza, Tatiane C de Carvalho, Luciana A Casemiro, Wilson R Cunha, Carlos H. G. Martins. Absence of the antibacterial activity of the crude extracts and compounds isolated from M. rubiginosa against extended-spectrum β-lactamase producing enterobacteria. pnr. 2011; 2(1): 1-7. doi: 10.4103/0976-9234.82982



Vancouver/ICMJE Style

Geisiany M de Queiroz, Maria Gorete M de Souza, Tatiane C de Carvalho, Luciana A Casemiro, Wilson R Cunha, Carlos H. G. Martins. Absence of the antibacterial activity of the crude extracts and compounds isolated from M. rubiginosa against extended-spectrum β-lactamase producing enterobacteria. pnr. (2011), [cited September 22, 2020]; 2(1): 1-7. doi: 10.4103/0976-9234.82982



Harvard Style

Geisiany M de Queiroz, Maria Gorete M de Souza, Tatiane C de Carvalho, Luciana A Casemiro, Wilson R Cunha, Carlos H. G. Martins (2011) Absence of the antibacterial activity of the crude extracts and compounds isolated from M. rubiginosa against extended-spectrum β-lactamase producing enterobacteria. pnr, 2 (1), 1-7. doi: 10.4103/0976-9234.82982



Turabian Style

Geisiany M de Queiroz, Maria Gorete M de Souza, Tatiane C de Carvalho, Luciana A Casemiro, Wilson R Cunha, Carlos H. G. Martins. 2011. Absence of the antibacterial activity of the crude extracts and compounds isolated from M. rubiginosa against extended-spectrum β-lactamase producing enterobacteria. Journal of Pharmaceutical Negative Results, 2 (1), 1-7. doi: 10.4103/0976-9234.82982



Chicago Style

Geisiany M de Queiroz, Maria Gorete M de Souza, Tatiane C de Carvalho, Luciana A Casemiro, Wilson R Cunha, Carlos H. G. Martins. "Absence of the antibacterial activity of the crude extracts and compounds isolated from M. rubiginosa against extended-spectrum β-lactamase producing enterobacteria." Journal of Pharmaceutical Negative Results 2 (2011), 1-7. doi: 10.4103/0976-9234.82982



MLA (The Modern Language Association) Style

Geisiany M de Queiroz, Maria Gorete M de Souza, Tatiane C de Carvalho, Luciana A Casemiro, Wilson R Cunha, Carlos H. G. Martins. "Absence of the antibacterial activity of the crude extracts and compounds isolated from M. rubiginosa against extended-spectrum β-lactamase producing enterobacteria." Journal of Pharmaceutical Negative Results 2.1 (2011), 1-7. Print. doi: 10.4103/0976-9234.82982



APA (American Psychological Association) Style

Geisiany M de Queiroz, Maria Gorete M de Souza, Tatiane C de Carvalho, Luciana A Casemiro, Wilson R Cunha, Carlos H. G. Martins (2011) Absence of the antibacterial activity of the crude extracts and compounds isolated from M. rubiginosa against extended-spectrum β-lactamase producing enterobacteria. Journal of Pharmaceutical Negative Results, 2 (1), 1-7. doi: 10.4103/0976-9234.82982