E-ISSN 2229-7723 | ISSN 0976-9234
 

Original Article 


The assessment of Cyclophosphamide chemotherapy effect loading-PLGA nanoparticles against ovarian cancer cells line (OVCAR-4 & PEO1)

Ali A. Majeed, Lina A. Hassan, Hydar muhsin Khalfa.

Abstract
Our study aimed to ameliorate the efficacy of anticancer drug of Cyclophosphamide on ovarian cancer cell lines related to human by loading with poly (D,L lactic-co-glycolic acid) (PLGA) which considered as nanoparticles (NPs) material. The merge of Cyclophosphamide – PLGA NPs were done by the technique of nanoprecipitation. NPs was prepared equal scattered with an average diameter of 133±25.19 nm. The dose response curves of free Cyclophosphamide dose were orgnized (12.5-2000 uM) by using the assay of MTT on OVCAR-4 and PE01 cell lines for 1 ,3 and 5 days. IC50 on OVCAR-4 was calculatrd and were 178 uM after one day of exposure , 15.3 uM and 1.38 uM after three and five days respectively. On the other hand, the IC50 on PE01 was only noticed after five days of exposure for Cyclophosphamide (11.70 uM). The viability of OVCAR-4 after treatment with Cyclophosphamide at 100 uM with free or with PLGA NPs consctruction for three days was 41.4% and 14.9% respectively. Resemblance, when 250uM was applied, the viability of OVCAR-4 was 19.2% and 11.8% after wxposure to free and NPs forms of Cyclophosphamide. In addition , the cell viability of PE01 when 250 uM applied ,the free or NPs constructions was 55.45% and 37.21% respectively. Oue work showed that OVCAR-4 cell line was more susceptible to Cyclo-PLGA NPs as compared to PE01 cells. conclusion : our action manifested that the effcet of Cyclophosphamide is time-depended and the protract effect originate by PLGA NPs may give a share in, at least to boost and increase efficacy of Cyclophosphamide as anticancer activity drug.

Key words: Ovarian cancer cells , PLGA-NP , Cyclophosphoamide


 
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How to Cite this Article
Pubmed Style

Majeed AA, Hassan LA, Khalfa Hm. The assessment of Cyclophosphamide chemotherapy effect loading-PLGA nanoparticles against ovarian cancer cells line (OVCAR-4 & PEO1). PNR. 2022; 13(1): 39-43. doi:10.47750/pnr.2022.13.01.008


Web Style

Majeed AA, Hassan LA, Khalfa Hm. The assessment of Cyclophosphamide chemotherapy effect loading-PLGA nanoparticles against ovarian cancer cells line (OVCAR-4 & PEO1). https://www.pnrjournal.com/?mno=17948 [Access: April 20, 2022]. doi:10.47750/pnr.2022.13.01.008


AMA (American Medical Association) Style

Majeed AA, Hassan LA, Khalfa Hm. The assessment of Cyclophosphamide chemotherapy effect loading-PLGA nanoparticles against ovarian cancer cells line (OVCAR-4 & PEO1). PNR. 2022; 13(1): 39-43. doi:10.47750/pnr.2022.13.01.008



Vancouver/ICMJE Style

Majeed AA, Hassan LA, Khalfa Hm. The assessment of Cyclophosphamide chemotherapy effect loading-PLGA nanoparticles against ovarian cancer cells line (OVCAR-4 & PEO1). PNR. (2022), [cited April 20, 2022]; 13(1): 39-43. doi:10.47750/pnr.2022.13.01.008



Harvard Style

Majeed, A. A., Hassan, . L. A. & Khalfa, . H. m. (2022) The assessment of Cyclophosphamide chemotherapy effect loading-PLGA nanoparticles against ovarian cancer cells line (OVCAR-4 & PEO1). PNR, 13 (1), 39-43. doi:10.47750/pnr.2022.13.01.008



Turabian Style

Majeed, Ali A., Lina A. Hassan, and Hydar muhsin Khalfa. 2022. The assessment of Cyclophosphamide chemotherapy effect loading-PLGA nanoparticles against ovarian cancer cells line (OVCAR-4 & PEO1). Journal of Pharmaceutical Negative Results, 13 (1), 39-43. doi:10.47750/pnr.2022.13.01.008



Chicago Style

Majeed, Ali A., Lina A. Hassan, and Hydar muhsin Khalfa. "The assessment of Cyclophosphamide chemotherapy effect loading-PLGA nanoparticles against ovarian cancer cells line (OVCAR-4 & PEO1)." Journal of Pharmaceutical Negative Results 13 (2022), 39-43. doi:10.47750/pnr.2022.13.01.008



MLA (The Modern Language Association) Style

Majeed, Ali A., Lina A. Hassan, and Hydar muhsin Khalfa. "The assessment of Cyclophosphamide chemotherapy effect loading-PLGA nanoparticles against ovarian cancer cells line (OVCAR-4 & PEO1)." Journal of Pharmaceutical Negative Results 13.1 (2022), 39-43. Print. doi:10.47750/pnr.2022.13.01.008



APA (American Psychological Association) Style

Majeed, A. A., Hassan, . L. A. & Khalfa, . H. m. (2022) The assessment of Cyclophosphamide chemotherapy effect loading-PLGA nanoparticles against ovarian cancer cells line (OVCAR-4 & PEO1). Journal of Pharmaceutical Negative Results, 13 (1), 39-43. doi:10.47750/pnr.2022.13.01.008