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ORIGINAL ARTICLE
Year : 2011  |  Volume : 2  |  Issue : 1  |  Page : 20-23

Insignificant difference seen on biofilm production among indwelling medical device associated bacterial isolates


1 Department of Microbiology, Government Medical College, New Rander Road, Surat, India
2 Department of Microbiology, Government Medical College, Bhatar Road, Surat, India

Correspondence Address:
Sangita B Revdiwala
28, Parikshit Society, opp Gajjar Park apartment, Besides Rupali canal, Bhatar road, Surat - 395 001
India
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Source of Support: None, Conflict of Interest: None


DOI: 10.4103/0976-9234.82988

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Aim of study : Biofilm formation is a developmental process with intercellular signals that regulate growth. Biofilms contaminate catheters, ventilators and medical implants; they act as a source of disease for humans, animals and plants. In this study we have done quantitative assessment of biofilm formation in device-associated clinical bacterial isolates in response to various concentrations of glucose in tryptic soya broth and with different incubation time. Materials and Methods: The study was carried out on 100 positive bacteriological cultures of medical devices which were inserted in hospitalized patients. The bacterial isolates were processed as per microtitre plate method with tryptic soya broth alone and with varying concentrations of glucose and were observed in response to time. Results: The majority of catheter cultures were positive. Out of the total 100 bacterial isolates tested, 88 were biofilm formers. Biofilm production was more in 0.25% than in 0.5% glucose concentration in tryptic soya broth. After 16-h incubation no significant difference was seen in biofilm production. Conclusions: Availability of nutrition in the form of glucose enhances the biofilm formation by bacteria, but further increase in glucose concentration could not enhance biofilm production. Biofilm formation depends on the adherence of bacteria to various surfaces.


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