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Journal of Pharmaceutical Negative Results
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Year : 2012  |  Volume : 3  |  Issue : 1  |  Page : 41-45

Insignificant antimicrobial activities and phytochemical screening in different extracts of indian Ginseng

1 Laboratory of Tissue Culture and Secondary Metabolites, Department of Botany University of Rajasthan, Jaipur, India
2 Veterinary Officer and In-charge, Goverment. Veterinary Hospital, Pahari (Bharatpur) Rajasthan, India

Correspondence Address:
Premlata Singariya
Dr. D. S. Kothari Post Doctoral Fellow, P.No.55, Shri Kalyan Nagar, Kartarpura, Jaipur-302015 (Rajasthan)
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Source of Support: None, Conflict of Interest: None

DOI: 10.4103/0976-9234.99665

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Aim: The aim of present study is to investigate the antimicrobial activity of (Flower, Ripen fruit and calyx) of Withania somnifera (RUBL20668) extracts, in order to use them as a possible source of new antimicrobial substances against important human pathogens. Materials and Methods: Crude extracts of different parts of both species of W. somnifera were evaluated against some important bacteria (two Gram +ve and four Gram-ve bacteria). Staphylococcus aureus (Gram +ve), Bacillus Subtilis (Gram +ve), Escherichia coli (Gram-ve), Raoultella planticola (Gram-ve), Pseudomonas aeruginosa (Gram-ve), Enterobactor aerogens (Gram-ve), one yeast Candida albicans and one fungus Aspergillus flavus. The dried and powdered seeds were successively extracted with hexane, toluene, iso propyl alcohol, acetone and ethanol, using the soxhlet assembly. The antimicrobial activity assay was done by both disc diffusion and serial dilution methods. Result: The results indicate that all the extracts in different polar solvents did not show any antibacterial activity against S. aureus, E. coli, R. planticola or E. aerogens or any antifungal activity against A. flavus. Conclusion: All extracts in the different polar solvents did not have or had very less antibacterial and antifungal activities.

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