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ORIGINAL ARTICLE
Year : 2015  |  Volume : 6  |  Issue : 1  |  Page : 33-36

Absence of anti-Staphylococcus aureus (MRSA) activity of secondary metabolite actinomycetes associated sponges from Pulau Panjang, Indonesia


1 Department of Microbiology, Faculty of Medicine, University of Indonesia, Jakarta, Indonesia
2 Laboratorium of Ecology and Zoology, Department of Biology, Faculty of Mathematic and Sciences, Sepuluh Nopember Institute of Technology, Surabaya, East Java, Indonesia
3 Department of Pharmacology, Faculty of Pharmacy, Pancasila University, Jagakarsa, Jakarta-Selatan, Indonesia

Correspondence Address:
Syamsudin Abdillah
Department of Pharmacology, Faculty of Pharmacy, Pancasila University, Jagakarsa, Jakarta Selatan, Jakarta
Indonesia
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Source of Support: None, Conflict of Interest: None


DOI: 10.4103/0976-9234.157388

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Objective: To study the anti-Staphylococcus aureus MRSA of the secondary metabolite actinomycetes associated sponges from Pulau Panjang, Central Java, Indonesia. Materials and Methods: The sponge samples were taken by scuba diving in Pulau Panjang, and the sponge was identified using external morphology as well as identification of spicules from the fresh preparation. Isolation of actinomycetes associated with sponge was conducted by means of direct plating. The symbion bacteria were planted (scratched in a circular way) on agar medium Zobell 2216E, medium actinomycetes using the existing method, namely, S.aureus MRSA and E. coli MRSA. Results: The results of identification from 6 sponges were Phakettia euctimenna, Gelliodes fibolatus, Lamellodysidea herbacea, Clathria reindwardtii and Callyspongia sp, while antibacterial assay for the secondary metabolite actinomycetes associated sponges showed negative results. Conclusion: Secondary metabolite actinomycetes associated sponges from Pulau Panjang were not found to have antibacterial activity against S. aureus MRSA.


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