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ORIGINAL ARTICLE
Year : 2017  |  Volume : 8  |  Issue : 1  |  Page : 2-6

Ultraviolet spectrophotometric method: Not possible for the simultaneous estimation of active constituents in Phyllanthus amarus


Oriental College of Pharmacy and Research, Oriental University, Sanwer Road, Jakhya, Opposite Revati Range Gate No. 1, Indore, Madhya Pradesh, India

Correspondence Address:
Rakhi Khabiya
Oriental College of Pharmacy and Research, Oriental University, Sanwer Road, Jakhya, Opposite Revati Range Gate No. 1, Indore, Madhya Pradesh
India
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Source of Support: None, Conflict of Interest: None


DOI: 10.4103/0976-9234.204909

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Objective: Our objective was to develop and validate UV spectrophotometric method for simultaneous estimation of phyllanthin, hypophyllanthin, and niranthin in Phyllanthus amarus. P. amarus is highly valued in the treatment of liver ailments and has been shown to posses antihepatitis B virus surface antigen activity. Several analytical methods including high-performance thin layer chromatography (HPTLC) and high-performance liquid chromatography (HPLC) are reported for respective phytoconstituents. However, there is no simultaneous estimation of phyllanthin, hypophyllanthin, and niranthin, active constituent of P. amarus. Hence, attempts have been made to develop UV spectrophotometric method because of the simplicity and economical advantage of this technique over other analytical techniques as HPTLC, HPLC, etc. Materials and Methods: Standard solution of phyllanthin, hypophyllanthin, and niranthin were prepared in methanol and scanned under a Shimadzu UV spectrophotomer, under full UV (ultraviolet) range (200-400 nm) to get the spectra. Result: After some experiments, it was found that it is impossible to quantify phyllanthin, hypophyllanthin, and niranthin simultaneously by UV spectrophotometric method. This was evident from the UV spectra as they were overlapping with each other. Conclusion: Same class of drugs may have almost the same functional groups, and gradient reverse-phase liquid chromatography and HPTLC will be more useful to separate such complicated mixtures. Therefore, we propose to develop either HPLC or HPTLC method for simultaneous estimation of these compounds.


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