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ORIGINAL ARTICLE
Year : 2017  |  Volume : 8  |  Issue : 1  |  Page : 31-36

Lack of in vitro anticancer and antimicrobial activities from Karanda (Carissa carandas) fruit extracts


Faculty of Science and Technology, Suan Sunandha Rajabhat University, Bangkok, Thailand

Correspondence Address:
Yuttana Sudjaroen
Faculty of Science and Technology, Suan Sunandha Rajabhat University, Bangkok
Thailand
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Source of Support: None, Conflict of Interest: None


DOI: 10.4103/jpnr.JPNR_5_17

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Background: Carissa carandas L. (Apocynaceae), commonly known as Karanda, is a widely used medicinal plant. In Thailand, Karanda fruits are favorite fruits especially in central region due to attractive shape and color with health promoting activities. Aims: To evaluate its antimicrobial and anticancer activities for claim promoting activities. Cytotoxicity of extracts was also evaluated with normal cells to claim for health safety. Materials and Methods: Anticancer activities of Karanda fruits extracted with dichloromethane (KD) and methanol (KM) were performed by resazurin microplate assay and tested with five cell lines, including KB-oral cavity cancer, MCF7-breast cancer, NCI-H187-small lung cancer, HepG2-hepatocarcinoma, and Caco2-colon adenocarcinoma cell lines. Cytotoxicity of KD and KM were performed as above and used HDFn-neonatal dermal fibroblast as normal cells. Antimicrobial activities of KD and KM against herpes simplex virus type I (HSV-1) and Mycobacterium tuberculosis were tested according by green fluorescent protein-based assay; Candida albicans were tested according to resazurin microplate assay, and Plasmodium falciparum K1 strain was tested according to microculture radioisotope techniques. Results: Both extracts were not possessed anticancer activity to any cancer cell lines at maximum concentration = 100 µg/mL. In the same way to anticancer activity assays, both extracts were not inhibited HSV-1, P. falciparum K1 strain, and M. tuberculosis (maximum concentration = 50 µg/mL). Also, both extracts were nontoxic to normal cells. Conclusion: KD and KM extracts of ripped Karanda fruits had no significant anticancer and antimicrobial activities with noncytotoxicity.


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