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LETTER TO THE EDITOR
Year : 2017  |  Volume : 8  |  Issue : 1  |  Page : 51-52  

Antibacterial efficacy after incorporation of CPP-ACP (GC Tooth MousseTM) into glass Ionomer cement against Streptococcus Mutans: A preliminary in vitro study


Department of Pedodontics and Preventive Dentistry, KLE VK Institute of Dental Sciences, KLE University, Belagaum, India

Date of Web Publication21-Apr-2017

Correspondence Address:
Niraj Gokhale
Department of Pedodontics and Preventive Dentistry, KLE VK Institute of Dental Sciences, KLE University, Belagaum, Karnatka
India
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Source of Support: None, Conflict of Interest: None


DOI: 10.4103/0976-9234.204907

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How to cite this article:
Gokhale N, Sood P, Hugar S, Shah P, Kohli D, Badkar C. Antibacterial efficacy after incorporation of CPP-ACP (GC Tooth MousseTM) into glass Ionomer cement against Streptococcus Mutans: A preliminary in vitro study. J Pharm Negative Results 2017;8:51-2

How to cite this URL:
Gokhale N, Sood P, Hugar S, Shah P, Kohli D, Badkar C. Antibacterial efficacy after incorporation of CPP-ACP (GC Tooth MousseTM) into glass Ionomer cement against Streptococcus Mutans: A preliminary in vitro study. J Pharm Negative Results [serial online] 2017 [cited 2017 Nov 19];8:51-2. Available from: http://www.pnrjournal.com/text.asp?2017/8/1/51/204907

Dear Sir

Casein Phosphopeptide- Amorphous Calcium Phosphate (CPP-ACP) are nano complexes which have been shown to prevent demineralization and promote remineralization.[1] CPP-ACP has been shown to interact with fluoride ions to produce an additive anti-cariogenic effect through formation of stabilized amorphous calcium fluoride phosphate phase.[2] Thus, this study was done to evaluate and compare the antibacterial efficacy of Glass Ionomer Cement, CCP-ACP (GC Tooth Mousse) incorporated into Glass Ionomer Cement, CCP-ACP (GC Tooth Mousse) against standard strains of Streptococcus Mutans(MTCC Number 25175)

A total of 9 samples were made with each of the three groups containing 3 specimens. The discs were prepared by using a standard hollow tube of diameter 0.5x1cm. The tube was pre sterilized to avoid contamination. For Group A, the Glass Ionomer cement was mixed as per the manufacturer's instruction to fill the hollow tube. The set material was retrieved and placed immediately into the BHI plate. For Group B specimens, the Glass Ionomer cement was mixed with 1 part of CPP-ACP paste (GC Tooth Mousse) and was allowed to set. The set material was then immediately transferred to the BHI plate. For Group C, the weight of the disc was determined and an equal quantity of the CPP-ACP paste (GC Tooth Mousse batch no. 15 117S) was dispensed onto the BHI plate by using a micropipette. [Figures 1]a-c BHI culture plates were inoculated with standard strains of Streptococcus Mutans (MTCC Number 25175) using lawn culture.
Figure 1: Preparation of specimen

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The plates were incubated at37°c and evaluated at regular intervals of 1st day, 3rd day, 7th day, 12th day [Figure 2] and the diameter of inhibition zone around each well was recorded in millimetres using a standard measuring scale
Figure 2: Zone of Inhibition at 1st , 3rd, 7th and 12th day

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No inhibition was seen in any of the groups. Even the conventional Glass Ionomers known to have an inhibitory effect on Streptococcus Mutans failed to show any inhibition. The addition of CPP-ACP to Glass Ionomer Cement did not have any additional effect on the inhibition. The time intervals at 1st day, 3rd day, 7th day, 12th day did not show any inhibition. The Glass Ionomer, GC Tooth Mousse™ and the combination of the two materials do not show any anti-microbial activity against standard strains of Streptococcus Mutans.

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Conflicts of interest

There are no conflicts of interest



 
   References Top

1.
Sunil S, Panchmal GS, Shenoy RP, Jodalli P, Sonde L., Caries prevention through casein phosphopeptide- amorphous calcium phosphate(CPP-ACP). Int J Oral Health Med Res 2015;2:70-3.  Back to cited text no. 1
    
2.
Mickenautsch S., Mount G.J., Yengopal V., Therapeutic effect of glass ionomers: An overview of evidence. Aust. Dent. J. 2011;56:10-5.  Back to cited text no. 2
    


    Figures

  [Figure 1], [Figure 2]



 

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