Advertisment ACS-IndiaSymposium
 
Journal of Pharmaceutical Negative Results
  Print this page Email this page Small font sizeDefault font sizeIncrease font size 
Search Article 
  
Advanced search 
 Home | About us | Editorial board | Search | Ahead of print | Current issue | Archives | Submit article | Instructions | Subscribe | Contacts  
 
ORIGINAL ARTICLE
Year : 2017  |  Volume : 8  |  Issue : 1  |  Page : 7-10

In vitro cytotoxicity evaluation of stem bark and leaves of Anogeissus pendula: Lack of potent activity


1 Department of Pharmacy, Banasthali University, Banasthali, Rajasthan; Department of Pharmacology, Khalsa College of Pharmacy, Amritsar, Punjab, India
2 Department of Pharmaceutical Chemistry and Analysis, Khalsa College of Pharmacy, Amritsar, Punjab, India
3 Department of Pharmacy, Banasthali University, Banasthali, Rajasthan, India

Correspondence Address:
Rakesh Yadav
Department of Pharmacy, Banasthali University, P.O. Banasthali University, Tonk, Rajasthan
India
Login to access the Email id

Source of Support: None, Conflict of Interest: None


DOI: 10.4103/0976-9234.204915

Rights and Permissions

Objective: Anogeissus pendula Edgew (Combretaceae) which is also known as “button tree” is one of the prominent species of Anogeissus genus which has several ethnomedicinal uses. Phytochemical investigation revealed the presence of alkaloids and phenolic compounds, such as flavonoids, lignins, tannins. So, pharmacological evaluation of this plant may lead to discovery of new activity. Therefore, the objective of the present study is to evaluate the in vitro cytotoxicity of stem bark and leaves hydroalcoholic extract of A. pendula. Materials and Methods: In vitro cytotoxicity was evaluated by MTT (3-(4,5-dimethyl thiazol-2-yl)-5-diphenyl tetrazolium bromide) assay against A431 (human epithelial carcinoma) and HepG-2 (human liver carcinoma) cell lines. The assay is based on the principle that the dead cells do not reduce the tetrazolium into formazan. Various concentrations (62.5-1000 μg/mL) of hydroalcoholic extracts were used and the concentration of test drug needed to inhibit cell growth by 50% (IC50) values is generated from the dose–response curves for each cell line. Microscopic examination was also carried out for morphological analysis of cells treated with extracts of A. pendula. Results: The hydroalcoholic extracts of stem bark and leaves revealed dose dependent, but insignificant activity against the cell lines i.e.A431 and HepG-2 as the IC50 was found to be greater than 1000 μg/mL for both the extracts against both the cell lines. There were no morphological changes in cells which supports the insignificant cytotoxicity. Conclusion: The results suggest that the hydroalcoholic extracts of stem bark and leaves of A. pendula have insignificant cytotoxicity.


[FULL TEXT] [PDF]*
Print this article     Email this article
 Next article
 Previous article
 Table of Contents

 Similar in PUBMED
   Search Pubmed for
   Search in Google Scholar for
 Related articles
 Citation Manager
 Access Statistics
 Reader Comments
 Email Alert *
 Add to My List *
 * Requires registration (Free)
 

 Article Access Statistics
    Viewed857    
    Printed11    
    Emailed0    
    PDF Downloaded130    
    Comments [Add]    

Recommend this journal