Journal of Pharmaceutical Negative Results

ORIGINAL ARTICLE
Year
: 2015  |  Volume : 6  |  Issue : 1  |  Page : 37--39

Assessment of aqueous extract of Lemon verbena on anxiety-like behavior in rats


Mojgan Veisi, Siamak Shahidi, Alireza Komaki, Abdolrahman Sarihi 
 Neurophysiology Research Center, Hamadan University of Medical Sciences, Hamadan, Iran

Correspondence Address:
Mojgan Veisi
Neurophysiology Research Center, Hamadan University of Medical Sciences, Hamadan
Iran

Aim: The purpose of the present study was to evaluate the anxiolytic effects of the aqueous extract of Lemon verbena on rats. Materials and Methods: In this study, aqueous extract of Lemon verbena leaves was prepared, and then male Wistar rats (200-230 g) were divided randomly into six experimental groups. Each group received a single intraperitoneal (IP) injection of saline, aqueous extract of Lemon verbena (10 mg/kg, 100 mg/kg, 500 mg/kg, 1,000 mg/kg)or diazepam, respectively. The level of anxiety was asserted by an elevated plus maze (EPM) 20 min after treatment. Statistical Analysis Used: Results were analyzed by one-way analysis of variance (ANOVA) and Tukey«SQ»s post-hoc test. The results were expressed as mean ± standard error of mean (SEM). The differences were considered significant at P < 0.05. Results: The results of this study showed that there was a significant difference in the number of entrances into the open arms, spent time in the open arms and number of total entrances into the open and closed arms of the EPM between control, diazepam and extract-receiving groups (10 mg/kg, 100 mg/kg, 500 mg/kg, 1,000 mg/kg) and control. The numbers of entrances into the open arms, spent time in the open arms in extract receiving groups were significantly less than control group. Conclusion: These results indicated that acute administration of aqueous extract of Lemon verbena (≥10 mg/kg) increased anxiety-like behavior in rats in the EPM.


How to cite this article:
Veisi M, Shahidi S, Komaki A, Sarihi A. Assessment of aqueous extract of Lemon verbena on anxiety-like behavior in rats.J Pharm Negative Results 2015;6:37-39


How to cite this URL:
Veisi M, Shahidi S, Komaki A, Sarihi A. Assessment of aqueous extract of Lemon verbena on anxiety-like behavior in rats. J Pharm Negative Results [serial online] 2015 [cited 2020 Jun 5 ];6:37-39
Available from: http://www.pnrjournal.com/article.asp?issn=0976-9234;year=2015;volume=6;issue=1;spage=37;epage=39;aulast=Veisi;type=0