Journal of Pharmaceutical Negative Results

ORIGINAL ARTICLE
Year
: 2016  |  Volume : 7  |  Issue : 1  |  Page : 12--15

Effect of topical gel prepared with hydroalcoholic extract of Echinacea purpurea on treatment of Leishmania major-induced cutaneous leishmaniasis in BALB/C mice


Bahador Sarkari1, Hossein Sattari2, Mahmoud Reza Moein3, Ali Mohammad Tamadon3, Reza Shahriari Rad4, Qasem Asgari5 
1 Basic Sciences in Infectious Diseases Research Center, Shiraz University of Medical Sciences, Shiraz, Iran
2 School of Pharmacy, Shiraz University of Medical Sciences, Shiraz, Iran
3 Department of Pharmacognosy and Medicinal Plants Research Center, Shiraz University of Medical Sciences, Shiraz, Iran
4 Student Research Committee, Shiraz University of Medical Sciences, Shiraz, Iran
5 Department of Parasitology and Mycology, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran

Correspondence Address:
Bahador Sarkari
Basic Sciences in Infectious Diseases Research Center, Shiraz University of Medical Sciences, P. O. Box: 71345-1735, Shiraz
Iran

Aim: This study aimed to investigate the effect of alcoholic extract of Echinacea purpurea as a topical gel for the treatment of Leishmania major-induced cutaneous leishmaniasis (CL) in BALB/c mice. Materials and Methods: Leishmania major was inoculated into the tail base of 28 BALB/c mice and the mice were then assigned into four groups, with seven mice in each group. Hydroalcoholic extract of Echinacea purpurea was prepared and 3% carboxymethylcellulose was used for the topical gels. After the development of skin lesions, the gel base without drug and 10% and 20% Echinacea gel, respectively, were applied for the treatment of skin lesions in three groups of mice for 20 days, two times per day. The forth group remained as the control without receiving any treatment. Sizes of the lesions were frequently measured and recorded. The collected data were analyzed by SPSS using the Kruskal-Wallis one-way analysis of variance test. Results: The sizes of the lesions at the tail base of BALB/c mice were found to be increasing in both the treated and untreated mice. Although there were differences in the mean size of the lesions between the control group and those that received various concentration of Echinacea gel, (10% or 20%) the differences were not statistically significant (P > 0.05). Conclusion: The results showed that topical 10% and 20% gel of Echinacea purpurea extract is not considerably effective in the treatment or control of CL.


How to cite this article:
Sarkari B, Sattari H, Moein MR, Tamadon AM, Rad RS, Asgari Q. Effect of topical gel prepared with hydroalcoholic extract of Echinacea purpurea on treatment of Leishmania major-induced cutaneous leishmaniasis in BALB/C mice.J Pharm Negative Results 2016;7:12-15


How to cite this URL:
Sarkari B, Sattari H, Moein MR, Tamadon AM, Rad RS, Asgari Q. Effect of topical gel prepared with hydroalcoholic extract of Echinacea purpurea on treatment of Leishmania major-induced cutaneous leishmaniasis in BALB/C mice. J Pharm Negative Results [serial online] 2016 [cited 2017 Jul 24 ];7:12-15
Available from: http://www.pnrjournal.com/article.asp?issn=0976-9234;year=2016;volume=7;issue=1;spage=12;epage=15;aulast=Sarkari;type=0