Journal of Pharmaceutical Negative Results

ORIGINAL ARTICLE
Year
: 2018  |  Volume : 9  |  Issue : 1  |  Page : 44--48

Lack of antibacterial activity of aqueous and ethanolic leaf extracts of Ziziphus talanai (Blanco) Merr.


Angelico Garcia Reyes1, Adrian Eustrace P Miclat2, Angelo B Bañares3, Renato A Dela Peña4 
1 Department of Natural Sciences and Mathematics, Institute of Arts and Sciences, Mabalacat City College, Mabalacat City, 2010 Pampanga; Biology Department, College of Science, De La Salle University, 2401 Taft Ave., Malate, Manila, 1004 Metro Manila, Philippines
2 Department of Natural Sciences and Mathematics, Institute of Arts and Sciences, Mabalacat City College, Mabalacat City, 2010 Pampanga; Department of Education, Division of Angeles City, Jesus St., Pulong Bulo, Angeles City, 2009 Pampanga, Philippines
3 Department of Natural Sciences and Mathematics, Institute of Arts and Sciences, Mabalacat City College, Mabalacat City, 2010 Pampanga, Philippines; Department of Energy Science and Technology, Energy and Environment Fusion Technology Center, Myongji University, Myongji-ro 116, Cheoin-gu, Yongin, Gyeonggi-do, Korea 170-58
4 Chemistry and Life Sciences Department, Manila Tytana Colleges, Pres. Diosdado Macapagal Blvd., Pasay City, 1300 Metro Manila, Philippines; Science Education Department, Br. Andrew Gonzalez College of Education, De La Salle University, 2401 Taft Ave., Malate, Manila, 1004 Metro Manila, Philippines

Correspondence Address:
Angelico Garcia Reyes
Biology Department, College of Science, De La Salle University, 2401 Taft Ave., Malate, Manila, 1004 Metro Manila
Philippines

Objective: The objective of the study is to investigate the phytochemicals and potential antibacterial activity of aqueous and ethanolic leaf extracts of Ziziphus talanai. Materials and Methods: Aqueous and ethanolic crude leaf extracts were subjected to phytochemical screening and assayed for their antimicrobial activities via paper disc diffusion method against Escherichia coli and Staphylococcus aureus. Results: Glycosides, condensed tannins, and saponins were found present in the two extracts; however, leucoanthocyanins were additionally detected in the aqueous extract. Both extracts exhibited no antibacterial activity against E. coli and S. aureus as indicated by the absence of a zone of inhibition. Conclusion: The results of the present study suggest a limited presence of phytochemicals in the crude aqueous and ethanolic leaf extracts of Z. talanai. Water can extract more phytochemicals from Z. talanai leaves than ethanol. However, the results further suggest that the phytochemicals from the leaves extracted using water and ethanol do not have antibacterial activities against E. coli and S. aureus. The negative results of the antimicrobial assay using the crude leaf extracts may not be generalized as lack of bioactivity of the other parts of Z. talanai.


How to cite this article:
Reyes AG, P Miclat AE, Bañares AB, Dela Peña RA. Lack of antibacterial activity of aqueous and ethanolic leaf extracts of Ziziphus talanai (Blanco) Merr. J Pharm Negative Results 2018;9:44-48


How to cite this URL:
Reyes AG, P Miclat AE, Bañares AB, Dela Peña RA. Lack of antibacterial activity of aqueous and ethanolic leaf extracts of Ziziphus talanai (Blanco) Merr. J Pharm Negative Results [serial online] 2018 [cited 2019 Sep 19 ];9:44-48
Available from: http://www.pnrjournal.com/article.asp?issn=0976-9234;year=2018;volume=9;issue=1;spage=44;epage=48;aulast=Reyes;type=0