Journal of Pharmaceutical Negative Results

ORIGINAL ARTICLE
Year
: 2019  |  Volume : 10  |  Issue : 1  |  Page : 25--31

Hibiscus sabdariffa linn fruits methanolic extract and fractions mediated glucose uptake stimulation and glucose transporter 4 regulation


Raheem Mohssin Shadhan1, Siti Pauliena Mohd Bohari1, Zainah Adam2, Haryati Jamaluddin3 
1 Department of Biotechnology and Medical Engineering, Faculty of Biosciences and Medical Engineering, Universiti Teknologi Malaysia, Johor Bahru, Johor, Malaysia
2 Medical Technology Division, Malaysian Nuclear Agency, Selangor, Malaysia
3 Department of Biosciences and Health Sciences, Faculty of Bioscience and Bioengineering, Universiti Teknologi Malaysia, Skudai, Malaysia

Correspondence Address:
Siti Pauliena Mohd Bohari
Department of Bioscience, Faculty of Science, Universiti Teknologi Malaysia, Johor Bahru 81310, Johor
Malaysia

Objective: As such, this study reports the effects of Hibiscus sabdariffa Linn fruit methanolic extract and fractions (ethyl acetate, n-hexane, and butanol) on glucose uptake stimulation, as well as regulation of glucose transporter-4 (GLUT4) in L6 myotubes and 3T3F442A adipocytes cells. Materials and Methods: The stimulating effects of methanolic extract and fractions of H. sabdariffa Linn fruit on glucose uptake in 3T3F442A adipocytes through in vitro assay assessment are reported. The GLUT4 translocation of L6 myotubes membrane was also investigated. Results: The outcomes of this study revealed that methanolic extract and fractions were unable to induce glucose uptake in 3T3F442A adipocytes. In addition, the methanolic extract and fractions were incapable of enhancing both the distribution and the regulation of GLUT4 in L6 myotubes, in relation to the control. Conclusion: The systematic empirical works performed in this study conclude that the H. sabdariffa Linn derivatives are unsuitable potentials for the progression of new anti-diabetic drugs.


How to cite this article:
Shadhan RM, Bohari SP, Adam Z, Jamaluddin H. Hibiscus sabdariffa linn fruits methanolic extract and fractions mediated glucose uptake stimulation and glucose transporter 4 regulation.J Pharm Negative Results 2019;10:25-31


How to cite this URL:
Shadhan RM, Bohari SP, Adam Z, Jamaluddin H. Hibiscus sabdariffa linn fruits methanolic extract and fractions mediated glucose uptake stimulation and glucose transporter 4 regulation. J Pharm Negative Results [serial online] 2019 [cited 2020 Jun 5 ];10:25-31
Available from: http://www.pnrjournal.com/article.asp?issn=0976-9234;year=2019;volume=10;issue=1;spage=25;epage=31;aulast=Shadhan;type=0