Journal of Pharmaceutical Negative Results

ORIGINAL ARTICLE
Year
: 2020  |  Volume : 11  |  Issue : 1  |  Page : 9--14

Lack of activity of rutin isolated from Tontelea micrantha leaves against Vero and BHK, fungi, bacteria and Mayaro virus and its in silico activity


Waleska Stephanie Da Cruz Nizer1, Ariane Coelho Ferraz2, Thais De Fatima Silva Moraes3, Fernanda Lopes Ferreira4, Cintia Lopes De Brito Magalhães2, Sidney Augusto Vieira-Filho5, Lucenir Pains Duarte4, José Carlos De Magalhães1 
1 Departamento de Química, Biotecnologia e Engenharia de Bioprocessos, Universidade Federal de São João del-Rei, São João del-Rei, Brazil
2 Departamento de Ciências Biológicas, Universidade Federal de Ouro Preto, Ouro Preto, Brazil
3 Departamento de Microbiologia, Universidade Federal de Minas Gerais, Belo Horizonte, Brazil
4 Departamento de Química, Instituto de Ciências Exatas, Universidade Federal de Minas Gerais, Belo Horizonte, Brazil
5 Departamento de Farmácia, Escola de Farmácia, Universidade Federal de Ouro Preto, Ouro Preto, Brazil

Correspondence Address:
Prof. José Carlos De Magalhães
Universidade Federal de São João del-Rei, Campus Alto Paraopeba, Rodovia MG 443, Km 7, CEP: 36420-000, Ouro Branco, MG
Brazil

Objectives: This work aimed to evaluate the antibacterial, antifungal, and anti-Mayaro virus (MAYV) activity of rutin, a flavonoid isolated from the leaves of Tontelea micrantha (Celastraceae). Materials and Methods: The antibacterial and antifungal activities were evaluated by the broth microdilution method through the determination of the minimum inhibitory concentration. The anti-MAYV activity was determined by the rutin concentration required to protect 50% of the cells after viral infection. The indirect 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide colorimetric assay was used to determine the cytotoxic concentration of rutin to 50% of Vero and BHK cells. The antimicrobial activity spectrum of rutin was predicted using the online software prediction of activity spectra for substances (PASS). Results: Although the PASS prediction had shown that a higher probability of rutin acts as an antifungal agent, the in vitro assays showed no antimicrobial activity. Regarding the cytotoxicity assay, rutin was not toxic to Vero and BHK cells. Conclusions: Many biological activities have been described for flavonoids, but the flavone rutin did not show antimicrobial activity in vitro. The results obtained in this work suggest that rutin is not a promising antimicrobial agent.


How to cite this article:
Nizer WS, Ferraz AC, Moraes TD, Ferreira FL, Magalhães CL, Vieira-Filho SA, Duarte LP, De Magalhães JC. Lack of activity of rutin isolated from Tontelea micrantha leaves against Vero and BHK, fungi, bacteria and Mayaro virus and its in silico activity.J Pharm Negative Results 2020;11:9-14


How to cite this URL:
Nizer WS, Ferraz AC, Moraes TD, Ferreira FL, Magalhães CL, Vieira-Filho SA, Duarte LP, De Magalhães JC. Lack of activity of rutin isolated from Tontelea micrantha leaves against Vero and BHK, fungi, bacteria and Mayaro virus and its in silico activity. J Pharm Negative Results [serial online] 2020 [cited 2020 Aug 4 ];11:9-14
Available from: http://www.pnrjournal.com/article.asp?issn=0976-9234;year=2020;volume=11;issue=1;spage=9;epage=14;aulast=Nizer;type=0