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  Indian J Med Microbiol
 

Figure 4: Effect of KYP-2047 on prolyl oligopeptidase (POP) activity within bloodstream forms of Trypanosoma brucei and human myeloid leukemia HL-60 cells. The cells were incubated with 100 μM KYP-2047 for 24 h, then harvested and washed, and finally lysates prepared to assay POP activity using the fluorogenic peptide substrate Suc-Gly- Pro-Leu-Gly-Pro-AMC. Data were calculated as specific activities (pmol AMC released/h) using a standard curve constructed with uncoupled AMC. The experiment was repeated 3 times and mean ± standard deviation are shown

Figure 4: Effect of KYP-2047 on prolyl oligopeptidase (POP) activity within bloodstream forms of <i>Trypanosoma brucei</i> and human myeloid leukemia HL-60 cells. The cells were incubated with 100 μM KYP-2047 for 24 h, then harvested and washed, and finally lysates prepared to assay POP activity using the fluorogenic peptide substrate Suc-Gly- Pro-Leu-Gly-Pro-AMC. Data were calculated as specific activities (pmol AMC released/h) using a standard curve constructed with uncoupled AMC. The experiment was repeated 3 times and mean ± standard deviation are shown