Bio Catalytic One-Pot Knoevenagel Condensation-Michael Type Addition-Heterocyclization Cascade For The Environmentally Friendly Synthesis Of Pyranopyrazoles In Non-Aqueous Media And Their Pharmacological Studies

Abstract

Using baker's yeast as a biocatalyst, the condensation of aromatic aldehydes, ethyl acetoacetate, malononitrile, and hydrazine hydrate yields an effective, environmentally friendly, and straightforward one-pot four-component synthesis of pyranopyrazole derivatives. These molecules were screened for pharmacological activities, and among the twelve derivatives, nitro substituent derivatives showed positive results and in docking results also showed more binding activity than all derivatives. Important aspects of the current methodology include the use of an environmentally friendly catalyst, high product yield, and mild reaction conditions, i.e. at neutral pH and ambient temperature.