Development And Validation Of Stability Indicating Rp-Hplc Method For Determination Of Chlorthalidone

Abstract

An RP-HPLC stability-indicating method was made and tested to find out how much Chlorthalidone is in both the bulk medicine sold in stores and the individual tablet lots. The chromatographic separation was performed at a flow rate of 1 ml/min at a measurement wavelength of 275 nm on an Agilent C18  column (250 x 4.6 mm, 5 m) using Water and Methanol (30/70 v/v) as the mobile phase. LOD and LOQ (Lower and upper limits of detection) as well as stability tests were performed on the method. The linearity was analyzed between 5 and 25 g/ml, and a correlation value of 0.990 was discovered. Both 0.40g/ml and 1.20g/ml were found to be the analytical and detection limits, respectively. Chlorthalidone was exposed to acidic, alkaline, oxidative, photolytic, and thermal breakdown stress conditions. Chlorthalidone is more affected by acidic conditions than by oxidation. It is less affected by alkaline conditions, heat, and light. The method is simple, reliable, sensitive, and accurate. It can tell the difference between the drug and its broken-down product that forms under different stress conditions. This allows for its application as a stability-indicating technique for locating CHD in both bulk and pharmaceutical dose form.