Scientific Validation Of Antihepatocellular Activity Of Terminalia Bellarica Seed Extracts Through In Vitro Cytotoxicity And Anticancer Activity Of HEPG2 Cells

Abstract

Terminalia bellirica (TB) is noted for its medicinal values and associated with many siddha, ayurvedic and unani preparations. It is commonly known as bahera or beleric and seemed to be a common plant on plains and hill areas especially, in Southeast Asia. The present study aimed to compare its cytotoxicity and anti carcinogenic activity of the seed extracts using Vero and HepG-2 (hepatoma) cell lines by MTT method.

T. bellerica seed was extracted with different solvents viz., methanol, ethylacetate, chloroform and aqueous. Among them, aqueous and chloroform extract activity was not confirmed with dose dependency on both of the cell lines, but, methanolic and ethylacetate extracts showed concentration dependent activities. Further, they were analysed on different concentration levels. The ethylacetate and methanolic extract showed IC⁵⁰ value as 20.11± 0.30, and 61.9 ± 0.30µg/ml respectively on HepG2 cells. Their activity was statistically significant and confirmed by evaluation of Hill coefficient method. The treated cells revealed the cell shrinkage and decrease in viable cells than the counterparts in controls. Further the findings of the study revealed that TB extract markedly possessed anticancer and anti-proliferative effects against hepatocellular carcinoma.