Spectrophotometric Determination Of Anti-Obesity Drug (Lorcaserin) By Azo Coupling Reaction With Brady’s Reagent

Abstract

The dedication of lorcaserin in its pure form and pharmaceutical preparations, with a simple, exact, precision, accurate and managed
spectrophotometric technique is studied. The azo coupling reaction of dinitrophenylhydrazine (2,4DNPH) reagent oxidized by
potassium iodate with lorcaserin in alkaline medium. The optimum conditions for the formation of the azo coupling dye were studied
from the impact of the reagent's extent and the volume of concentrated H2SO4, concentration and volume of oxidizing agent KIO3,
volume of NaOH, the quick response of time and the temperature effect. This method obeys Beer's law with concentrations ranging
from (25- 500) µg. mL-1
at max wavelength 590 nm. The Calculated values from the current study were LOD = 0.523 µg. mL-1
, LOQ
= 1.743 µg. mL-1
, linearity coefficient = 0.9990, sandals sensitivity =0. 294µg.cm-2
and stabilization constant is 5. 1×108
.The proposed
method has been successfully applied for the dedication of lorcaserin in its pharmaceutical preparations.