Evaluation of Interleukin-6 and Interleukin-10 in Patients with Urinary Tract Infection

Abstract

Background: Urinary tract infection (UTIs) are considered to be the most common infections in humans.
Aims: this study aimed to evaluate the immune status of the patients with UTI by studying of (IL-6 and IL-10).
Subjects and Methods: A total of 49 UTI patients infected with gram –ve bacteria and 25 healthy controls were enrolled in this study. Urine samples from UTI cases was collected in AL-Imam AL-Hussain teaching hospital in Thi- Qar province, during the period from February 2014 to July 2014. Urinary isolates were identified by conventional methods.
3 ml were collected in plastic tube and then centrifuged at 3000 round per minute (rpm) for 5 min to obtain sera. And after the serum sample were collected they were divided into two apendroff tubes and stored in deep freeze at – 20º C until performing IL-6 and IL-10. (Lewis et al., 2006).
Results: Patients with E. coli infection accounted for 26 (53.06%); other culture results were as follows: 7 (14.29 %) patients had K. pneumonia, 9 (18.37%) patients had P. aerogenosa, 4 (8.16%) had Proteus and 3 (6.12%) patients had K.oxytoca. Measure levels of serum IL-6 and IL-10 for study group against control group, which is showed a significant increase (P ≤ 0.01) in the levels of interleukins (IL-6, IL-10) in all serum patients compared to the control group.
Conclusion: E- coli was most common causative agent in UTI, The high rate concentration of IL-6 and IL-10 in patients with UTI compared with the healthy control group. This refers to the injury for their role in the immune response.