Synthesis, Characterization, Molecular Docking and Biological studies on Novel Pyazole fused Indole Derivatives

Abstract

The present work deals with the sequence of Novel Pyrazole fused Indole derivatives(4a-4l) were synthesized by simple conventional method and
screened for anthelmintic, antibacterial and anticancer activities. The molecular docking studies were also performed. All of the newly synthesized
compounds were structurally characterized on the basis of IR, 1HNMR and Mass spectral analysis. Further, all of the newly prepared pyrazole fused
indole derivatives were screened for anthelmintic activity by using Albendazole as standard drug. The antibacterial activity was carried out by agar
diffusion (Cup plate) method by using Streptomycin as standard and anticancer activity against MCF-7 cell lines by MTT assay method. The results
showed that some of the compounds 4b, 4c and 4f exhibited good anticancer activity. The compounds 4c, 4f, 4j showed good anthelmintic activity and
4b,4h and 4i exhibited potential antibacterial activity by comparing with standard drug. Additionally, the molecular docking studies of novel pyrazole
fused Indole derivatives was also carried out to explain putative bonding interaction between the active site of EGFR enzyme and synthesized Novel
Pyrazole fused Indole derivatives by Schrodinger suite.