DESIGN DEVELOPMENT AND CHARACTERIZATION OF OMEPRAZOLE LOADED NANOSUSPENSION

Abstract

The purpose of this study was to develop omeprazole nanosuspension with enhanced solubility and bioavailability; it was prepared by precipitation ultrasonication method. To ensure the quality of the omeprazole nanosuspensions, the selected preliminary trial formulation (F10) with particle size 198.6nm, entrapment efficiency 88.9% and in vitro drug release 82.2% at 60 min) was subjected to 2² factorial design.  The optimum composition obtained using a 2-factor, 2-level Factorial design was as follows: polyvinyl alcohol (90 mg), sonication time of 40 min.  The constant regression values for particle size was 175nm, zeta potential -24.5mV, in vitro drug release 87.3%, entrapment efficiency 92.7%. From the data it was observed that R2 formulation was the best formulation.Scanning Electron Microscopy revealed that the particles were prismatic in shape. Stability studies performed for a period of 3 months indicated that there were no significant changes in the in vitro drug release pattern and entrapment efficiency.