VITAMIN C REDUCES THE METANIL YELLOW INDUCED OXIDATIVE STRESS IN OVARIAN TISSUES OF RAT

Abstract

Metanil yellow (MY) is a banned synthetic azo dye that induces oxidative stress in rat ovarian tissue. To investigate the mitigating role of vitamin C in MY-induced oxidative stress in ovaries, the activity of antioxidant enzymes in ovarian tissue homogenates and cytoarchitectural changes in ovarian tissue sections of MY with vitamin C-treated rats were investigated. Female Charles Foster rats (age 90-120 days, weight 110-120 grams) bred in the laboratory were used in the study. The study found no significant changes in the production of superoxide dismutase, catalase, glutathione peroxidase, glutathione reductase, glutathione-s-transferase and malondialdehyde (MDA) in groups of rats that received MY with vitamin C compared to control rats. In addition, we found no significant structural changes in ovarian tissue in rats that received MY with vitamin C compared to control rats. The results suggest that vitamin C exerts a protective effect against MY-induced oxidative stress in ovarian tissues, probably by antagonizing MY-induced reduction of antioxidant enzyme activity, MDA production through lipid peroxidation, and stress-induced damage of ovarian tissues.