Investigation Of In-Vitro Antihelminthic Activity Using Ethanolic & Aqueous Extract Of Origanum Majorana Plant Leaves

Abstract

Research on novel medications is important since the majority of commercially available anti- helminthic products have problems with the emergence of helminth resistance. Most of the nearby plants have medical properties, making them a natural alternative source for gastrointestinal nematode anti-helminth drugs. Examining the chemical makeup of Origanum majorana plant leaves and determining their anti-helminthic effectiveness are the study & main goals. In this work, chemical components from the leaves of the Origanum majorana plant are extracted using both aqueous and alcoholic methods. An earthworm called Pheritima posthuma is employed to carry out in-vitro anthelmintic activities since it shares the same anatomical characteristics. Furthermore, it shares a physiological resembling traits with the human gastrointestinal roundworm.The earthworms, which were almost the same size, were dispersed into the 1 ml of the chemical formulation made from plant extracts in groups of around six apiece. The time it took for paralysis and death to occur was measured if there was no movement or very little movement, excluding when the worms were violently shaken. The earthworms were said to be paralysed when there was no movement seen other than when they were shook ferociously. The findings were recorded for each concentration based on the paralysis time and death time. The major evidence for this was the lack of movement in the worms in hot water or when shaken. Their motility may be observed to completely reduce or stop at zero when they die, and this is followed by a change in body colour. The anthelmintic activity of the Majaorana leaves & ethanolic and aqueous extracts was dose- or concentration-dependent, requiring the lowest amount of time to cause paralysis (P) and death (D) at a concentration of 150 mg/ml. Therefore, Origanum aqueous and ethanolic extracts, at various extraction concentrations, demonstrate their anthelmintic activity.