Efficiency Of Wharton's Jelly Mesenchymal Stem Cells With Saccharum Officinarum Juice And Glycyrrhiza Glabra Extract In Modulation Of Histopathological And Physiological Changes During Gastric Wound Healing In Rats

Abstract

Objective : Peptic ulcer disease means some kind of benign damage to the mucosa and the lining of the digestive tract. There is usually a link between gastric acid secretion and this injury, but nowadays, the leading cause of these ulcers is the presence and proliferation of Helicobacter pylori bacteria. Given the effects of chemical drugs, medicinal plants, and cell therapy as an alternative to treating diseases seems to be appropriate.

Methods: Twenty-four male Wistar rats weight 300±2 g randomly divided into six groups(n=4), control, peptic ulcer (PE) by Acetic acid, PE+ Saccharum officinarum juice as drinking water (n=4), PE+ Glycyrrhiza glabra extract (Gge) 200 mg/kg, PE+ distilled water as gavage control, PE+ Gge 200 mg/kg+ Soj + Wharton's jelly stem cells (WJMSCs). After 14 days, all groups evaluated and compared factors such as histopathological studies, weight and acidity changes, and changes in blood serum cytokines.

Results: According to the results of this study, the groups receiving Soj and Gge had a significant difference in the mentioned factors Compared to the control group (P˂0.05). Although WJMSCs, when combined with Soj and Gge, had a significant effect on inflammatory factors and body weight changes (P˂0.05), they were able to increase the thickness of the stomach wall.

Conclusion : These results suggest that Soj and Gge have given their compounds, which can be considered an alternative to gastric ulcer treatment, which can be accelerated and more efficient with cell therapy.