Estimation Of Coq10 In Bulk And Formulation By High Performance Thin Layer Chromatography

Abstract

High performance thin layer chromatography (HPTLC) gives many advantages over HPLC. It reduces the total cost of analysis as compared to HPLC. The mobile phase consumption per sample is extremely low in HPTLC, hence reducing the acquisition and disposal cost. Considering the cost and suitability of analysis for estimation of CoQ10 in bulk and its marketed capsule formulation, HPTLC method was developed and validated. The Camag HPTLC system, employed with software win CATS was used for proposed analytical work. Planar chromatographic development was carried out with the help of Pre-coated silica 60F254 TLC plates. Sample application was facilitated by Linomat 5 applicator. After sample application plates were subjected for ascending development in twin trough chamber of 10x10 cm dimension, using 10 ml of solvent system. The optimized mobile phase was composed of Toluene: ethyl acetate: chloroform (10:1:1 v/v/v). In post development, the plates were air dried and then scanned densitometrically using a UV detector at 550 nm in absorbance mode. In HPTLC densitogram well defined peak was obtained for CoQ10 with optimal Rf value of 0.71. Performance characteristics of HPTLC method for estimation of CoQ10 in bulk and its marketed capsule dosage form were statistically validated as per recommendations of ICH guidelines of analytical method validation. The HPTLC method was found to be linear across the range 50-250 ng/band. The LOD and LOQ values were found to be 0.0480 and 0.1467 µg respectively. The method was found to be accurate, precise, robust and economical for the analysis of CoQ10 from bulk and its capsule formulation.