Quantitative Analysis Of CD133 As A Prognostic Marker In Oral Epithelial Dysplasia

Abstract

Aim: To evaluate the expression of CD133 in Oral epithelial dysplasia.

Materials and methods: The study included a total of 50 samples of Archival tissue blocks that were categorised as control and cases (Oral epithelial dysplasia) and further as the cases were categorised again as mild, moderate and severe. The sections of both Group I and group II were subjected to Immunohistochemical staining using CD133 antibody. The cells were counted and the data obtained were compared for the significance keeping p<0.05 as significant.

Results: When compared with normal mucosa, the expression of CD133 increased in oral epithelial dysplasia. The mean number of positive cells was increased from Mild epithelial dysplasia to severe epithelial dysplasia. Severe epithelial dysplasia showed increased expression when compared with moderate and mild epithelial dysplasia. Comparison of different grades of epithelial dysplasia showed highly statistical significance. Comparison between and within the group showed highly statistical significant. Comparison of epithelial dysplasia with controls with mean number of positive cells with highly statistically significant.

Conclusion: The quantitative expression of CD133 increased from normal mucosa to the through the various differentiations of epithelial dysplasia, suggesting that CD133 might be involved for identification of cell proliferation from mild to severe epithelial dysplasia.