Development And Validation Of Stability Indicating HPLC Method For Estimation Of Pyrazinamide In Plasma

Abstract

Objectives:  A selective, precise and accurate  HPLC stability indicating assay  method  has  been  developed  for  the  simultaneous  estimation  of  pyrazinamide. 

Materials and  Methods:  The  efficient  chromatographic  separation  of  drug  was  achieved  by  using  Luna C-18 column. The mobile phase composition of 50:50 v/v acetonitrilel: buffer with a flow rate of 1 mL/min with detection wavelength of 285nm. 

Results:  The retention time and the linearity of pyrazinamide was found to be 3.49min and   the linearity range was found to be 50-300 ng/mL respectively. There were no changes in the retention time was observed. The precision of the drug denoted by %RSD was 8.00%  respectively.

Conclusion:  The  validation  parameters resulted that the  drug  product was efficiently  validated  from  the plasma  and  developed  a method.