Biofilm Formation And Antibiotic Resistance For E. Coli Isolated From Urinary Tract Infections From Iraqi Women With The Polycystic Ovarian Syndrome

Abstract

Background: Urinary Tract Infections (UTIs), particularly in Iraqi women, are among the most corporate bacterial illnesses. Objectives: The study's goal is to identify the most frequent bacterial type in Iraqi women with PCOS and UTI, as well as to assess these bacteria's ability to form a biofilm with resistance to antibiotics. Method: mid-stream urine specimens a total of 159 were collected randomly from Iraqi women at Baghdad in Al-Yarmouk teaching hospital separated into four groups, PCOS with UTI, PCOS without UTI, UTI without PCOS, and control group. Urine was cultured on (blood & MacConkey) agar, so VITEK2 compact system was used to identify purified colonies and used two methods for detection of biofilm formation. Results: out of 159, 60 women with PCOS and UTI or UTI without PCOS, 53.21% were positive growth, 86.2%, 13.8 was gram-negative and gram-positive respectively, and gram-negative bacteria were more prevalent, especially E. coli is the most common uropathogen in PCOS with UTI 42.8% and 44.4% for UTI without PCOS, the major of E. coli was sensitive to Imipenem and Meropenem (100%), Amoxicillin/clavulanic acid, Erythromycin (100%) resistance, Levofloxacin, Amikacin, Ciprofloxacin 33.3% respectively, Gatifloxacin 28.6%, Nitrofuratonin 19.1%, Fosfomycin 9.5%. CRA method showed 24% strong ability for E. coli biofilm formation, and 47%,29% for moderate and non-formation respectively. while the tissue microtiter plate method showed 52.4%, 9.5%, 28.6%, and 9.5% for strong, moderate, weak, and no biofilm formation respectively. Conclusion: Gram-negative bacteria were higher than Gram-positive isolates from both groups (PCOS with UTIs and UTIs without PCOS), as well as E. coli was the most common bacterial isolate, the highest level of resistance of E. coli was against both Erythromycin and Amoxicillin/clavulanic acid, whilst completely sensitive of E. coli was against Meropenem and Imipenem. biofilm detection by two methods, CRA method current results showed (52.4%) as strongly positive, while in M.T.P method about (52.4%) a strong ability for biofilm. In the menstrual cycle the irregular menstruation among the study groups PCOS, PCOS & UTI group is higher than the regular menstrual However, the acne factor in the skin of Iraqi women with Hirsutism, the abnormal hair density growth with PCOS, PCOS with UTI group was higher than UTI group, as well as Married women infected PCOS with and without UTI were significantly higher than single women respectively.