Screening Of Some Neuropharmacological Activities Of Naringi Crenulata (Roxb.)

Abstract

Objective: The goal of this study is to examine the neuropharmacological effects of various extracts made from Naringi crenulata (Roxb.).

Materials and methods: Wayanad, Kerala was the source of the leaves used in the authentication of Naringi crenulata (Roxb.). Petroleum ether, chloroform, and methanol were used as solvents with higher polarity throughout the extraction process. To obtain ethyl acetate fractions, the methanol extract was also separated with ethylacetate. All of the extracts and the fraction underwent a phytochemicals screening to determine their phytoconstituent composition. For various in- vitro antioxidant assay methods, including the DPPH scavenging assay, hydroxyl radical scavenging assay, nitric oxide radical scavenging assay, different concentrations (5–640 g/mL) of chloroform, methanol, and ethylacetate fractions were used. The standard was ascorbic acid (AA).On higher and lower doses of NC, neuropharmacological screening techniques were carried out as sedative and hypnotic activity, anti-convulsant activity. Catalase, SOD, GPx,(enzymatic) and non-enzymatic antioxidants(GSH) were used in the in-vivo antioxidant research on isolated liver. In MENC and EAFNC, the levels of the brain neurotransmitters GABA and DA were also examined. Additionally, the methanol extract underwent partial purification using column chromatography and was then submitted to GC-MS to determine whether bioactive chemicals were present.

Results: The phytochemical analysis of Naringi crenulata (Roxb.) revealed the presence of alkaloids, phenolic compounds, carbohydrates, and flavonoids. According to in-vitro antioxidant data, as extract concentration increased, the scavenging property also improved. This was followed by an increase in percentage inhibition, which demonstrated lower IC₅₀ values across the board for all extracts. With MENC, the greatest scavenging effect was discovered in testing techniques. Therefore, when compared to other antioxidants, this extract was thought to be beneficial. The increased dose of MENC was found to be significant when compared to other groups in in-vivo antioxidant activities, brain neurotransmitter estimate, and neuropharmacological screening procedures. A GC-MS analysis of the MENC partly purified column fractions revealed the presence of 26 bioactive chemicals, some of which had therapeutic potential.

Conclusion: The findings showed that Naringi crenulata (Roxb.) extracts can be employed as an antioxidant and in the treatment of  some  neurological illnesses.