Extraction. Isoaltion, Purification,Stability Studies And Cytotoxic Studies Of Phycocyanin From Spiruline Culture

Abstract

The aim of work is focus on Extraction. Isoaltion, putification of phycocynin from spirulina culture. In this method extraction was done by centrifuge process by using the buffer and saturated amonium sulphate, form crude extract purification done by using the ammonium sulfate extract using DEAE-Cellulose was used for anion exchange chromatography. The spectral charrecterzation was done by using IR,NMR and Mass Spectroscopy. Phycocyanin concentration and purity were determined by spectrophotometry using absorbance at 620 nm and 652 nm the purity was foune to be between 08-4.3 for diffent extractions like Crude extract ,Ammonium sulphate precipitation with 25% saturation ,Ammonium sulphate precipitation with 50% saturation, DEAE-cellulose-52. The stabilty studies for phycocyanin was perfomed ar different temperature and pH: 4 °C, 25 °C, and − 20 °C and pH: 5, 6, and 7.the phycocyanin content decreased to 15% at 25 °C, 83% at 4 °C, and 51% at − 20 °C. The most appropriate storage condition was 4 °C at pH 5. It was found that the most appropriate storage temperature at pH 6 was − 20 °C for better preservation of phycocyanin. We found that the most appropriate pH was 7, at − 20 °C. the cytotoxicity studies were performed for crude extract and crude Phycocyanin, pure phycocyanin were evaluated by the MTT assay against the HT-29 (colon cancer), MCF-7 (breast cancer) and DU-145 (prostate cancer) cell lines. Of all the three components tested against HT-29 cell lines, the pure Phycocyanin shows 135± 2 than the standard Methotrexate.  Of all the three components tested against MCF-7 cell lines, the pure Phycocyanin shows 155± 3 than the standard Methotrexate. Of all the three components tested against DU-145 cell lines, the pure Phycocyanin shows 174 ± 2 than the standard Methotrexate. Based on the above results it concludes that the pure Phycocyanin shoes better cytotoxicity against the HT-29 (colon cancer), MCF-7 (breast cancer) and DU-145 (prostate cancer) cell lines.