GREEN SYNTHESIS OF SILVER NANOPARTICLES USING Rosmarinusofficinalis LEAF EXTRACT AND STUDY OF ANTICANCER EFFECT AND APOPTOSIS INDUCTION ON PROSTATE CANCER CELL LINE (PC-3)

Abstract

Introduction: Rosmarinusofficinalis consist of many medicinal values like for the treatment of disorders associated with nervous,
cardiovascular, gastrointestinal, menstrual, hepatic and reproductive systems. The main aim is to synthesize silver nanoparticles using
Rosmarinusofficinalisleaf extract and to study the anti-cancer and apoptosis induction on prostate cancer cell line
Materials and method: The plant was collected, pressed and was made into powder. The prostate cancer cell line was purchased and was
subcultured in the laboratory. The cell line and different concentrations of drugs were subjected to MTT assay and an IC-50 value was found.
The prostate cancer cells RNA were isolated and were under gene expression. The RNA was made into cDNA using reverse transcriptase
which was then subjected to real time polymerase chain reaction for specific genes which promotes anti-cancer and apoptosis induction.
Results: The analysis of MTT assay showed that the IC-50 where only 50 percent of the cell was alive to certain concentrations of dosage
of the drug used. In this study, it was found out that 200 microgram of concentration was enough to reduce 50 percent of cells in a cell
culture. The genes caspase 3 and caspase 9 were analyzed and showed significant increase at 200 microgram of the drug
Rosmarinusofficinalis.
Conclusion: Rosmarinusofficinalis has a significant role in controlling prostate cancer cell line proliferation by upregulating the gene
expression. The results showed that R.officinalis exert cytotoxic activity on prostate cancer cell line