In vitro pharmacological investigations of Biophytum sensitivum callus extract: Lack of potent activities

Abstract

Objective: Biophytum sensitivum is an important medicinal plant extensively used in
traditional oriental herbal medicines. Though medicinal use of this plant is known, the
active principles responsible such property is not known. Pharmacological screening of
this plant may lead to discovery of new activity with new mode of treatment. Hence,
screening for in‑vitro pharmacological activities of methanolic callus extracts of Biophytum
sensitivum has been carried out. Experiments were designed according to the standard
methods and processes. Materials and Methods: Leaf cutting derived callus on MS
medium supplemented with BA (Benzyl adenine) 1.0 mg/l + NAA (1‑naphthaleneacetic
acid) 1.0 mg/l is used as a source, and compounds were extracted from dried callus
using methanol solvent with Soxhlet apparatus. Results: The callus extract has shown
antioxidant activity, in‑vitro inhibition of enzyme activities like α‑glycosidase, acetyl
cholesterase, and tyrosinase, but potency was found to be low. The Graph pad Prism
Version-5 software is used to analyze data in the form of Figures. Conclusion: For
the first time, we are reporting in‑vitro pharmacological screening of methanolic callus
extracts of Biophytum sensitivum.