Insignificant effect of cleistanthin A and cleistanthin B on motor function in animal models

Abstract

Aim: To study the in vitro and in vivo effects of cleistanthin A and cleistanthin B on
the cholinergic receptors and the motor function using mouse as an animal model.
Materials and Methods: The cleistanthin A and cleistanthin B were isolated from
the leaves of Cleistanthus collinus using column chromatography and purified. The
effect of cleistanthins A and B on locomotor activity, motor co‑ordination and grip
strength in mice were evaluated using actophotometer, rota‑rod apparatus and grip
strength test respectively (in vivo). The effect of cleistanthins A and B on nicotinic
acetylcholine receptor was studied using a mouse esophagus preparation (in vitro) and
the concentration‑response curves of carbachol and cleistanthins A and B were recorded
using a data acquisition system (Biopac Inc. USA) through a variable transducer (500
g). Results: Cleistanthins A and B did not have any significant effect on locomotor
activity, motor co‑ordination and grip strength when they were used upto 400 mg/kg
BW. Cleistanthins A and B did not show any significant inhibitory effect on nicotinic
acetylcholine receptors in isolated mouse esophagus tissue preparation at 1, 3, and
10 μg doses. Conclusion: Cleistanthins A and B do not have significant activity on
the motor system when used upto 400 mg/kg in mice and also they did not have any
effect on nicotinic acetylcholine receptors when used upto 10 μg in the isolated mouse
esophagus preparation.