Cytotoxic Effects Of Gallic Acid In Human Breast Cancer Cell Mda-Mb-231

Abstract

Background: Gallic acid is a naturally occurring phenolic molecule with antitumor effects on most forms of cancer. The impact of Gallic acid on MDA-MB-231 breast cancer cells was investigated in the current study.

Methods: The cytotoxic effects of Gallic acid on MDA-MB-231 cells were investigated using caspase-8&9 activity and high content screening assays. Apoptosis mechanisms following Gallic acid treatment were also investigated.

Results: Gallic acid have had greatest cytotoxic impact on MDA-MB-231 cells at a half-maximal inhibitory concentration (IC50) of 43.86 μg/mL. When MDA-MB-231 cells were treated with 200 μg/mL of Gallic acid, a substantial increase in caspase-8&9 activity was also detected. In addition, Gallic acid (200 μg/mL) increased cell membrane permeability, cytochrome c level, and nuclear intensity compared to untreated cells. Gallic acid (200 μg/mL) treatment resulted in a significant reduction in cell viability and mitochondrial membrane permeability compared to untreated cells serving as the negative control.

Conclusions: High-content screening and caspase-8&9 activity tests indicated that Gallic acid was cytotoxic to MDA-MB-231 cells in vitro. Gallic acid has shown as a potential chemopreventive agent for triple-negative breast cancer.