Formulation And Anti Inflammatory Studies Of Newly Formulated Arishta From Ficus Religiosa Root Bark

Abstract

Introduction: Ficus religiosa is a traditional medicinal plant used as a remedy for several ailments. Arishta is one of the unique and valuable therapeutic alignment, due to its efficacy, stability and its desirable features.

Methods: This study is to formulate an arishta from hydro alcoholic extract of Ficus religiosa root bark and then formulated arishta was estimated by a validated HPTLC method. Pharmacological evaluation of arishta was also done for evaluating its anti-inflammatory activity. The extraction was done by using different solvents like, hexane, petroleum ether, chloroform, ethanol and water, by soxhlet extraction method. Arishta was prepared as per Ayurvedic pharmacopoeia. HPTLC studies were carried out by using CAMAG HPTLC system and mobile phase used was toluene: ethyl acetate: formic acid: methanol (14:10:2:1). Pharmacological evaluation was done for finding its anti inflammatory activity by SRBC membrane stabilization method and Carrageenan induced paw oedema method.

Results: Arishta was prepared from Ficus religiosa root bark.  And HPTLC study helps to identify the marker compounds present in the formulated arishta of Ficus religiosa root bark. The proposed HPTLC method was found to be simple, precise, accurate, robust etc. and is suitable for estimation of herbal formulations. Formulated arishta at a concentration of 800µg/ml shows a good protection activity in SRBC membrane stabilization method and a concentration of 2ml/kg shows anti inflammatory activity in Carrageenan induced paw oedema method.

Conclusion: Arishta was prepared from hydro alcoholic extract of Ficus religiosa root bark, which shows a good anti inflammatory activity compared to NSAIDS, and HPTLC studies showed presence of tannins and phenolic compounds as marker compounds.