Hepatoprotective And Antioxidant Study Of Ethanolic Extract Of T. Cordifolia Against Cyclophosphamide Induced Liver Injuries

Abstract

The aim of study is to evaluate the hepatoprotective and antioxidant potential of the ethanolic extract of stem of T. cordifolia against anticancer drug cyclophosphamide. Cyclophosphamide (CPP) is an immunosuppressive agent, generally prescribed in treatment of various types of cancer, autoimmune disorders and also to stabilise the organ transplantations. The major setback of the CPP is that it undergoes extensive hepatic metabolism and transformed into active metabolites 4-hydroxycyclophosphamide, phosphoramide mustard and acrolein. Cyclophosphamide and its produced metabolites altogether alter the hepatocellular membrane permeability by initiating lipid peroxidation and leads hepatic injuries. Present study was conducted to establish the hepatoprotective potential of ethanolic extract of stem of Tinospora cordifolia extract (ETCE) against CPP induced hepatotoxicity. Six groups of Wistar rats (n=6) were constituted.  CPP intoxicated animals were treated with ETCE at dose of 250mg/kg, 500mg/kg and 1000mg/kg. Blood samples were collected on 14^th day of study and examined for liver function parameters (SGOT, SGPT, ALP and total bilirubin content). A significant (p<0.001) fall in the SGOT, SGPT, ALP and total bilirubin content were reported in dose dependent manner of ETCE. Liver homogenates were investigated for antioxidant effect of the ETCE against CPP induced oxidative stress.  A significant (p<0.001) antioxidant effect at dose of 500mg/kg and 1000mg/kg of ETCE were also reported as decrease in lipid peroxidation along with an increase in GSH, SOD level and catalase activity. Histopathological investigations of liver sections of animals supplemented with ETCE confirmed the restoration of normal hepatic architecture.