A SURGE IN ANTI-BACTERIAL AND ANTI-INFLAMMATORY ACTIVITY OF SEPIA PHARAONIS INK EXTRACT IN DIFFERENT SOLVENTS-AN IN-VITRO STUDY

Abstract

Aim & Objective: To analyse the anti-bacterial and anti-inflammatory action of Sepia pharaonis ink extract using various solvents. To test the anti-bacterial activity of SPIE using microbial culture. To evaluate the anti-inflammatory action of SPIE using Egg albumin denaturation assay.

Methodology: The Sepia pharaonis fish was procured from kasimedu coastal area. The SPIE was lyophilized into a black powder. One gram of lyophilized SPIE powder was mixed individually with DMSO, Ethanol and water respectively in an Eppendorf tube. The anti-bacterial action of SPIE was performed using Culture plate coated with Mullein Hinton agar. E. faecalis, S. Mutans, S. Aureus, Lactobacillus were the different bacteria used in the study. The Anti-Inflammatory action was performed using the Egg albumin denaturation assay technique and different concentrations of SPIE mixed solvents were used.

Result: The Anti-inflammatory and anti-bacterial action of Sepia pharaonis ink extract proved to be very effective in DMSO solvent followed by ethanol solvent and at last with water solvent. The anti-inflammatory action of SPIE mixed with DMSO was effective in 30 µL,40 µL and 50 µL Concentration compared to the standard drug. The anti-bacterial action of S.Mutans was very effective followed by E.faecalis and  Lacto-bacillus and  the action against S.Aureus was found to be minimal.

Conclusion: This study proves that SPIE can be used in the anti-inflammatory drugs in the treatment of patients with tumours. Antibacterial activity holds good in the formation of ointments, soaps and various cosmetics