Evaluation Of Antihyperlipidemic And Phytochemical Screening Of Vitex Trifolia Linn And Corchorus Trilocularis In Rat

Abstract

Aim & Objective: Effect of Antihyperlipidemic and Phytochemical Screening of Vitex Trifolia Linn and Corchorus Trilocularis In Rat

 Material & Method: Fresh leaves (immature, mature and senescence) were collected, air dried and the crude powder was prepared for phytochemical and antioxidant analysis. The powder was mixed with 70% methanol and the supernatant was separated. The antioxidant activity of this ethanolic extract was measured on the basis of the scavenging activity 1, 1-diphenyl 2-picrylhydrazyl (DPPH) free radical with slight modifications. Phytochemical analysis was performed according to standard laboratory protocol. Screening of antihyperlipidemic was done by using Corchorus trilocularis and Vitex trifolia linn. Leaves part extracts used for antihyperlipidemic activity leaves (200mg/kg and 400mg/kg). Hyperlipidemia was administered in experimental animals using high fat diet. In order to collect blood for high fat diet-induced hemolysis, heart punctures were performed after 14 days hyperlipidemia. Antihyperlipidemic activity of Corchorus trilocularis and Vitex trifolia linn plant extracts were evaluated by estimation of lipid profiles and standard drug Atorvastatin for 14 days.

 Results: The results indicated the presence of different phytochemicals viz. glycosides, steroids, cholesterol, alkaloids, phenols, flavonoids, riboflavin, saponins and terpenoids. The sample also showed antioxidant activity by inhibiting DPPH radical. The significant antioxidant activity of methanolic leaf extract of might be due to the presence of saponins, phenols, flavonoids and alkaloids found in the preliminary phytochemical analysis. Estimation of lipid profile shows that Corchorus trilocularis and Vitex trifolia linn leaves extract (200mg/kg and 400mg/kg) shows the less significant antihyperlipidemic activity. It decreases TC, TG, LDL, VLDL and increases HDL levels. Corchorus trilocularis and Vitex trifolia linn leaves extract 200mg/kg and 400mg/kg shows significant antihyperlipidemic activity as standard drug Atorvastatin.

 Conclusion: Present study reveals that the jute leaf possesses different phytonutrients and exhibited DPPH radical scavenging activity, and therefore, may be used for therapeutic purposes According to the phytochemical screening  it reveal that the hyperlipidemic effects of Corchorus trilocularis and Vitex trifolia linn (200mg/kg and 400mg/kg) are similar to the effect of standard drug Atorvastatin.