Evaluation And Association Of Periodontal Status With Level Of P. Intermedia In Chronic Periodontitis Female Patients With And Without Oral Contraceptive Pills Following Non-Surgical Periodontal Therapy Using Quantitative Polymerase Chain Reaction: An Int

Abstract

Aim : The aim of the present study was to detect and correlate the levels of Prevotella Intermedia   with  clinical  parameters  after  nonsurgical  periodontal  therapy (NSPT)  in  chronic  periodontitis female  patients  with  or without oral contraceptive pills, using quantitative polymerase chain reaction (Q‑PCR) method.

Materials and Methods : Sixty female  patients equally divided into three groups,

i.e. Periodontally health female patients (Group –I), females with  Chronic Periodontitis (CP) without taking oral contraceptive pills(OCP) (Group-II), females with  Chronic Periodontitis (CP)  taking oral contraceptive pills(OCP) (Group-III) and were correlated for the presence of P. Intermedia  in the respective groups. PPD, CAL, and saliva samples for microbiological evaluation  were  assessed  at  baseline,  1‑,  and  3‑month  post‑NSPT.

Results : Significant  reduction  of  PPD  was  found  2.4 ± 0.48mm versus 1.4 ± 0.37 mm in Group I, 4.8 ± 0.74 versus 2.8 ± 0.53 mm in Group II, and 6.6 ± 0.48 mm versus 4.4 ± 0.39 mm in Group III post‑NSPT at 3 months. Similarly, a notable reduction of CAL was exhibited  in both Group II (5.4 ± 0.65 vs. 3.1 ± 0.42 mm) and Group III (6.6 ± 0.48 vs 3.6 ± 0.35 mm) patients after NSPT at 3 months. A greater reduction of P. Intermedia concentrations was observed in both Group II and Group III at 3‑month post‑NSPT.

Conclusion : The substantial improvement of clinical parameters was found to be in  correlation with the load of P. Intermedia, which was reduced more in Group II than in Group III, emphasizing the applicability and sensitivity of Q‑PCR method for its assessment.