Development And Evaluation Of Lovastatin Transdermal Delivery System Using Polymeric And Solid Lipid Nanoparticles

Abstract

The main aim of our study is the incorporation of polymeric nanoparticles and solid lipid nanoparticles containing Lovastatin into transdermal patches. In our study, The aim of our study mainly supports for the prevention of first pass metabolism and achieve the controlled release.By using various phosphate buffer solutions, solubility studies were conducted and based upon their results phosphate buffer of pH 6.8 with 1% Tween 20 was selected as a medium for  in  vitro  studies.  By  using  FT-IR,  compatibility  studies   were   performed   between   the   drug   Lovastatin   and   excipients   used   in   the   formulation. In the nanoparticulated formulation there was no interaction between the drug and the excipients was shown by the compatibility studies. Solvent evaporation method was employed for the preparation of PLNs by using Chitosan, PLA, and PCL as polymers. Micro emulsion technique was employed for the preparation of  SLNs  by  using Stearic acid, cholesterol and Glyceryl monostearate as lipids. The prepared PLNs (SP1 to SP12) and SLNs (SL1 to SL6) formulations were evaluated in case of various parameters like morphology, particle size, zeta potential, PDI and in vitro drug release  and the results of the above parameters were observed to be in the desired range,With different concentrations of HPMC, the formulations SP4 and SL6 were selected and incorporated into transdermal patch. Evaluation for the various parameters was done for the prepared patches (TPN1 to TPN3 and SLNP1 to SLNP3) and the results were recorded individually. Based on the results obtained TPN1 and SLNP2 were selected for formulating the transdermal patches by incorporating different permeation enhancers. Evaluation was done for the transdermal formulations (PLP1 to PLP12 and SLP1 to SLP12) and the results were recorded individually. PLP12 (Span 80 as permeation enhancer) and SLP9 (DMSO as permeation enhancer) were observed to be the best formulation and were selected and subjected to in vivo analysis separately and were compared with and without permeation enhancers patches. These final patches showed a significant decrease in the serum cholesterol, LDL, VLDL & triglycerides levels and  have increased the HDL and total protein levels significantly.