Evaluation Of Anticancer, Anti-Inflammatory And Antioxidant Activity Of The Aerial Parts Of Mollugo Verticillata Using MCF-7 Cell Line

Abstract

Mollugo verticillata aerial parts have been used as a folk remedy to treat fever, wounds, sores, and swelling, and to alleviate pre mensural symptoms (PMS). As a result, the purpose of this study is to investigate the antioxidant, anti-inflammatory, and anticancer activity of Mollugo verticillata aerial parts. To obtain the extracts, the aerial parts were subjected to successive extractions with water and ethanol. The antioxidant activity was measured using the H2O2 scavenging assay, the reducing power assay, and the ABTS free radical scavenging assay. The anti-inflammatory activity was determined by inhibiting the protein denaturation assay. MTT assay was used to assess the plant's in vitro cell cytotoxicity activity, and the AO/PI dual staining method was used to assess the plant's apoptotic effect. The phytochemical composition of aqueous and ethanolic extracts of the plant showed the presence of flavonoids, saponins, glycosides, phytosterols, coumarin, tannins, and phenols. Our results revealed that Mollugo verticillate extracts have strong antioxidant activity against free radicals. The anti-inflammatory activity of ethanolic extract was found to be 95.098% at a concentration of 150mg/mL with an IC50 value of 54.68g/mL, while the aqueous extract's was found to be 83.333% with an IC50 value of 83.36g/mL. The MTT assay and AO/PI dual staining results showed that the plant extract induced apoptosis and had promising cytotoxic effects in MCF-7 cancer cells. The current study's findings have provided some information on phytochemical and pharmacological properties.