Effect Of Fertilization Of Bio-Rhizobacterein, Nano-NPK And Nano-Micro Complete On The Enzymatic Activity Of Bean Rhizosphere

Abstract

A factorial experiment was carried out in the wooden canopy of Agriculture and Forestry College / University of Mosul during the winter season on 15/11/2021, to study the effect of bio-fertilizer Rhizobacterein, Nano-NPK 20:20:20 and Nano-micro complete with the time periods on the activity of urease and phosphatase enzyme using the soil of Hawi el-mosul region with (Silt Loam) texture. The experiment included two factors, the first factor included nine treatments of Nano-NPK20:20:20, Nano-micro complete fertilizers , Rhizobacterein biofertilizer, traditional fertilizer, doul and triple treatments. The second factor included the time periods (60, 90 and 120) days after of cultivation according to the complete random design (CRD) with three replicates. The original Italian bean (Luz) seeds were planted on 24 November, 2021, in ten kg soil of plastic. Two batches of fertilizer were applied to the soil combined with germination, the first batch 20 days later, and the second batch 20 days later. For all treatments, the efficacy of each urease and phosphatase enzyme was assessed, for the planting periods (60, 80, and 120) days after cultivation.

    The results of the statistical analysis of LSD_0.05 showed that the tri combination of fertilization (Nano-NPK 20:20:20 + Nano- Micro Complete + Bio- Rhizobacterein) for the periods 60, 90 and 120 days after cultivation was superior to the activity of the urease enzyme (120.00, 60.70 and 48.70) μg N-NH₄ gm^-1 soil 2 hr^-1 respectively, followed by the same combination of regular sources for the same time periods (83.30, 41.00, and 34.80) μg N-NH₄ gm^-1  soil 2 hr^-1 respectively, While the same trend was achieved with the activity of the phosphatase enzyme for the tri-nano combination (430.9, 320.1, and 277.00) µg PNP gm^-1 soil 1 hr^-1 respectively, compared to the conventional mixture and for the same periods (348, 235.2, and 135.00) µg PNP, 1 gm^-1 soil 1 hr^-1, sequentially.