Studies On Optimization Of Parameters For Extraction Of Total Phenolic Content From Terminalia Arjuna

Abstract

Terminalia arjuna is commonly known as Arjun tree and belongs to Combretaceae family. It has a high phytochemical content and functions as an antioxidant. It exhibits a variety of pharmacological effects, including those against cancer, free radicals, ulcers, diabetes, and helminths. In this study, the total phenolic content (TPC) of Terminalia arjuna's leaves and bark was extracted by optimising physico-chemical parameters such as the impacts of various solvents, solvent percentages, pH, extraction time, and particle mesh size. The best extraction conditions for TPC from bark were determined to be ethanol solvent, 60% solvent, 48 hours of extraction duration, pH 6, 72 mesh size, and a concentration of 25.0 g/ml. Additionally, methanol solvent, 100% solvent, 48 hours of extraction time, pH 8, 72 mesh size, and a concentration of 21.5 g/ml were shown to produce the best results for the extraction of TPC from leaves. The best extraction conditions for TPC were determined to be methanol solvent, 80% solvent, 24 hours of extraction duration, pH 6, 72 mesh size, and a concentration of 27.0 g/ml.